Helicobacter pylori interferes with leukocyte migration via the outer membrane protein HopQ and via CagA translocation

International Journal of Medical Microbiology : IJMM
Benjamin BuschRainer Haas

Abstract

The human gastric pathogen Helicobacter pylori is a paradigm for chronic bacterial infections. Persistent colonization of the stomach mucosa is facilitated by several mechanisms of immune evasion and immune modulation, such as avoidance of Toll-like receptor recognition or skewing of effector T cell responses. Interactions of H. pylori with different immune cells have been described with respect to immune cell activation, cytokine release, or oxidative burst induction. We show here that H. pylori infection of human granulocytes, or of HL-60 cells differentiated to a granulocyte-like phenotype (dHL-60 cells) results in inhibition of cell migration under different conditions. Migration of dHL-60 cells in a three-dimensional collagen gel was found to be inhibited independently of the cag pathogenicity island, whereas migration inhibition in an under agarose assay was dependent on the cag pathogenicity island, on its effector protein CagA, and on the outer membrane protein HopQ. CagA translocation into leukocytes is accompanied by its tyrosine phosphorylation and by proteolytic processing into an N-terminal 100 kDa and a C-terminal 35 kDa fragment at a distinct cleavage site. By using complemented H. pylori strains producing either...Continue Reading

References

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S J CollinsR C Gallo
May 17, 2001·Antimicrobial Agents and Chemotherapy·J BylundC Dahlgren
Jul 16, 2002·Infection and Immunity·M InnocentiM Quiding-Järbrink
Nov 7, 2002·Molecular Cell·Hitomi MimuroChihiro Sasakawa
Feb 20, 2003·Science's STKE : Signal Transduction Knowledge Environment·Bryan Heit, Paul Kubes
Jan 20, 2004·Journal of Bacteriology·Wolfgang Fischer, Rainer Haas
Jul 24, 2004·Infection and Immunity·Katharina KranzerWulf Schneider-Brachert
Dec 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Motohiro KobayashiMinoru Fukuda
Jul 8, 2005·FEMS Microbiology Letters·Mikael BrisslertMarianne Quiding-Järbrink
Mar 18, 2006·The Journal of Clinical Investigation·Amedeo AmedeiMarina de Bernard
Aug 24, 2006·Helicobacter·Masanori Hatakeyama, Tomasz Brzozowski
Nov 15, 2006·Infection and Immunity·Peter MitchellGiovanna Lombardi
Mar 29, 2007·Cellular Microbiology·Andre Dubois, Thomas Borén
Oct 9, 2007·FEMS Immunology and Medical Microbiology·Holly M Scott AlgoodTimothy L Cover
Jan 16, 2009·The FEBS Journal·Alessandro AngeliniDarren J Hart
Apr 8, 2009·Infection and Immunity·Sarah E SzarowiczFrederick S Southwick
Apr 22, 2009·Cell Host & Microbe·Matthias SelbachMatthias Mann
May 6, 2009·Infection and Immunity·Helena SvenssonMarianne Quiding-Järbrink
Jun 16, 2009·Current Opinion in Cell Biology·Tim Lämmermann, Michael Sixt
Aug 26, 2009·Helicobacter·Vittorio NecchiEnrico Solcia
Dec 8, 2009·Nature Structural & Molecular Biology·Dragana NesićC Erec Stebbins
Mar 20, 2010·Mucosal Immunology·D BimczokL E Smythies
Apr 27, 2013·Pathogens and Disease·Jessica Shiu, Thomas G Blanchard
May 9, 2013·Nature Reviews. Microbiology·Nina R SalamaAnne Müller

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthrax Vaccines (ASM)

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.

Anthrax

Anthrax toxin, comprising protective antigen, lethal factor, and oedema factor, is the major virulence factor of Bacillus anthracis, an agent that causes high mortality in humans and animals. Here is the latest research on Anthrax.

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.

Babesiosis

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Atrophic Gastritis

Atrophic Gastritis is a process where gastric glandular cells are lost and replaced with firbous tissues, as a result of chronic inflammation. Learn more about Atrophic Gastritis here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Anthrax Vaccines

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.