Helicobacter pylori lipopolysaccharide promotes a Th1 type immune response in immunized mice

Vaccine
Jennifer M TaylorJay V Solnick

Abstract

Helicobacter pylori (H. pylori) infection is prevalent worldwide and results in chronic gastritis, which may lead to peptic ulcer disease or gastric cancer. The goal of this study was to determine the role that H. pylori lipopolysaccharide (LPS) plays in stimulating host immune responses in the context of a vaccine. We compared H. pylori SS1 sonicate (LPS+) to a sonicate depleted of LPS (LPS-) in immunized BALB/c mice. Naïve splenocytes produced high levels of TNF-alpha and IL-10 after incubation with LPS+ sonicate, while cells incubated with LPS- sonicate did not. Mice immunized with LPS+ sonicate developed a prominent innate response characterized by increased TNF-alpha and IL-10, as well as a strong antigen specific Th1 response including, IFN-gamma, IL-2 and high IgG2a serum titers. Mice that received LPS- sonicate were strongly Th2 biased in their immune response, with significantly more IL-4 than IFN-gamma and serum IgG1 titers higher than IgG2a. Together these studies suggest that H. pylori LPS in a whole cell sonicate vaccine promotes a Th1 immune response that may aid in protection or clearance of H. pylori infection.

References

Jul 1, 1994·The Journal of Infectious Diseases·H NielsenA P Moran
Mar 1, 1997·Scandinavian Journal of Gastroenterology·J PiotrowskiB L Slomiany
Feb 17, 1999·The Journal of Experimental Medicine·S T QureshiD Malo
Mar 21, 2002·Nature Reviews. Cancer·Richard M Peek, Martin J Blaser
Oct 11, 2002·The New England Journal of Medicine·Sebastian Suerbaum, Pierre Michetti
May 23, 2003·Infection and Immunity·Christine A GarhartSteven J Czinn
Jul 10, 2004·Trends in Molecular Medicine·Mario M D'EliosGianfranco Del Prete
Sep 24, 2004·Microbiology and Immunology·Toshiro YamamotoJiro Imanishi
Dec 15, 2004·Microbes and Infection·Katherine A FitzgeraldDouglas T Golenbock

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Citations

Jul 23, 2013·Journal of Clinical Biochemistry and Nutrition·Kenji FurutaYoshikazu Kinoshita
Dec 17, 2008·Hematology·Roberto Stasi, Drew Provan
May 19, 2009·European Journal of Cell Biology·Natasa ObermajerJanko Kos
Mar 27, 2007·Best Practice & Research. Clinical Gastroenterology·Karen RobinsonJohn C Atherton
Jun 13, 2012·Scandinavian Journal of Immunology·F Martínez-BecerraY López-Vidal
Mar 17, 2009·The American Journal of Pathology·Benny L W WongHui Yao Lan
Apr 11, 2008·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·T MoujaberG L Gilbert
Dec 2, 2010·The Korean Journal of Hematology·Hee Sang TagYang Soo Kim
Apr 20, 2007·FEMS Immunology and Medical Microbiology·Ann-Mari Svennerholm, Anna Lundgren
Aug 12, 2014·World Journal of Gastroenterology : WJG·Magdalena ChmielaKarolina Rudnicka
Sep 19, 2014·World Journal of Gastroenterology : WJG·Florian Anderl, Markus Gerhard
Jun 6, 2017·Critical Reviews in Microbiology·Nasrin MirzaeiHajieh Ghasemian Safaei
Sep 23, 2021·Expert Review of Vaccines·Henderson ZhuAndrew J Pollard

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