Helicobacter pylori Might Induce TGF-β1-Mediated EMT by Means of cagE

Helicobacter
Hyun ChangDong Ho Lee

Abstract

Epithelial-mesenchymal transition (EMT), in which polarized epithelial cells have mesenchymal cell phenotypes, is thought to be a key process of invasion and metastasis of cancer. Transforming growth factor beta-1 (TGF-β1) is known to be carcinogenic and Helicobacter pylori is a predominant carcinogen of gastric cancer. Our study aimed to determine whether TGF-β1 or H. pylori infection enhances EMT process and cytotoxin-associated gene E (CagE) is associated with EMT. Human gastric cancer cell AGS and MKN45 were treated with recombinant TGF-β1 or H. pylori including cagE-negative (ΔcagE) mutant. Besides the assessment of EMT-related markers expression levels by means of RT-qPCR, Western blot, and immunofluorescence assay, the induction of in vitro EMT on gastric cancer cells (AGS and MKN cell lines) was confirmed by wound-healing assay and invasion assay. When gastric cancer cells were treated with TGF-β1 or various strains of cagE-positive H. pylori, EMT-related marker altered significantly. However, the ΔcagE mutant did not. Wound-healing assay and invasion assay showed enhanced migration ability of the cells treated with cagE-positive H. pylori but not in ΔcagE mutant. EMT induction in gastric cancer cells by TGF-β1 was conf...Continue Reading

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Citations

Nov 20, 2015·Frontiers in Cellular and Infection Microbiology·Nianshuang LiNong-Hua Lu
Sep 4, 2015·Cellular Microbiology·Trevelyan R MenheniottAndrew S Giraud
Dec 23, 2015·The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi·Sang Hwan LeeKi Baik Hahm
Mar 5, 2019·Journal of Gastroenterology and Hepatology·Nayoung Kim
Mar 5, 2016·World Journal of Gastroenterology : WJG·Jing ZhongLiang-Jing Wang
Jun 6, 2017·Critical Reviews in Microbiology·Nasrin MirzaeiHajieh Ghasemian Safaei
Sep 2, 2020·International Journal of Molecular Sciences·Carla FiorentiniAlessia Fabbri

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