Helicobacter pylori protein response to human bile stress

Journal of Medical Microbiology
Chunhong ShaoJihui Jia

Abstract

The ability of Helicobacter pylori to tolerate bile is likely to be important for its colonization and survival in the gastrointestinal tract of humans. As bile can be acidified after reflux into the low pH of the human stomach, the inhibitory effect of fresh human bile with normal appearance on H. pylori before and after acidification was tested first. The results showed that acidification of bile attenuated its inhibitory activity towards H. pylori. Next, the protein profiles of H. pylori under human bile and acidified bile stress were obtained by two-dimensional electrophoresis. Protein spots with differential expression were identified using tandem matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The results showed that the changes in proteomic profiles under bile and acidified bile stress were similar when compared with that of normal H. pylori. Expression of 28 proteins was found to be modulated, with the majority being induced during bile or acidified bile exposure. These proteins included molecular chaperones, proteins involved in iron storage, chemotaxis protein, enzymes related to energy metabolism and flagellar protein. These results indicate that H. pylori responds to bile and acidified ...Continue Reading

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Citations

Sep 19, 2009·The Journal of Microbiology·Wei QuJihui Jia
Dec 3, 2010·Omics : a Journal of Integrative Biology·Dimiter V Dimitrov
Aug 13, 2013·PLoS Pathogens·Christopher L NoblesAnthony W Maresso
Nov 13, 2010·FEMS Immunology and Medical Microbiology·Yinghui ZhaoJihui Jia
Mar 17, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Raksawan DeenonpoeBanchob Sripa
Oct 9, 2013·Molecular Medicine Reports·Chunhong ShaoJihui Jia

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