Helicobacter pylori Type IV Secretion System and Its Adhesin Subunit, CagL, Mediate Potent Inflammatory Responses in Primary Human Endothelial Cells

Frontiers in Cellular and Infection Microbiology
Mona TafreshiTerry Kwok

Abstract

The Gram-negative bacterium, Helicobacter pylori, causes chronic gastritis, peptic ulcers, and gastric cancer in humans. Although the gastric epithelium is the primary site of H. pylori colonization, H. pylori can gain access to deeper tissues. Concurring with this notion, H. pylori has been found in the vicinity of endothelial cells in gastric submucosa. Endothelial cells play crucial roles in innate immune response, wound healing and tumorigenesis. This study examines the molecular mechanisms by which H. pylori interacts with and triggers inflammatory responses in endothelial cells. We observed that H. pylori infection of primary human endothelial cells stimulated secretion of the key inflammatory cytokines, interleukin-6 (IL-6) and interleukin-8 (IL-8). In particular, IL-8, a potent chemokine and angiogenic factor, was secreted by H. pylori-infected endothelial cells to levels ~10- to 20-fold higher than that typically observed in H. pylori-infected gastric epithelial cells. These inflammatory responses were triggered by the H. pylori type IV secretion system (T4SS) and the T4SS-associated adhesin CagL, but not the translocation substrate CagA. Moreover, in contrast to integrin α5β1 playing an essential role in IL-8 inductio...Continue Reading

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Citations

Jun 22, 2018·Future Microbiology·Xiao-Yan YuanMing-Yi Wang
Sep 12, 2018·Helicobacter·Langgeng Agung WaskitoYoshio Yamaoka
Jul 11, 2019·Cell Communication and Signaling : CCS·Wang-Yu CaiQi-Cong Luo
Oct 3, 2018·Helicobacter·Alice BlosseAlain P Gobert
Mar 17, 2020·Journal of the American Heart Association·Xiujuan XiaCanxia Xu
May 16, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Wongwarut BoonyanugomolWoo-Kon Lee

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Methods Mentioned

BETA
scanning electron microscopy
nuclear translocation
electrophoresis
ELISA
PCR
PCRs
by scanning electron microscopy

Software Mentioned

LinRegPCR
GraphPad
Adobe Photoshop
Prism

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