Helicobacter pylori upregulates expression of epidermal growth factor-related peptides, but inhibits their proliferative effect in MKN 28 gastric mucosal cells

The Journal of Clinical Investigation
M RomanoR Zarrilli

Abstract

Acute exposure to Helicobacter pylori causes cell damage and impairs the processes of cell migration and proliferation in cultured gastric mucosal cells in vitro. EGF-related growth factors play a major role in protecting gastric mucosa against injury, and are involved in the process of gastric mucosal healing. We therefore studied the acute effect of H. pylori on expression of EGF-related growth factors and the proliferative response to these factors in gastric mucosal cells (MKN 28) derived from gastric adenocarcinoma. Exposure of MKN 28 cells to H. pylori suspensions or broth culture filtrates upregulated mRNA expression of amphiregulin (AR) and heparin-binding EGF-like growth factor (HB-EGF), but not TGFalpha. This effect was specifically related to H. pylori since it was not observed with E. coli, and was independent of VacA, CagA, PicA, PicB, or ammonia. Moreover, H. pylori broth culture filtrates stimulated extracellular release of AR and HB-EGF protein by MKN 28 cells. AR and HB-EGF dose-dependently and significantly stimulated proliferation of MKN 28 cells in the absence of H. pylori filtrate, but had no effect in the presence of H. pylori broth culture filtrates. Inhibition of AR- or HB-EGF- induced stimulation of cel...Continue Reading

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Citations

Jan 2, 2007·Diseases of the Colon and Rectum·Marco RomanoFrancesco Selvaggi
Oct 13, 2000·Gastroenterology Clinics of North America·M F Go, S E Crowe
Mar 14, 2001·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·V RicciM Romano
Apr 3, 2002·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·V RicciM Romano
Sep 19, 2002·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·C TuccilloM Romano
Oct 28, 2006·Nature Clinical Practice. Gastroenterology & Hepatology·Marco RomanoRaffaele Zarrilli
May 25, 2010·Nature Reviews. Cancer·D Brent Polk, Richard M Peek
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Jan 28, 2014·Seminars in Cell & Developmental Biology·Carmen Berasain, Matías A Avila
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Sep 27, 2005·The Journal of Pathology·Concetta TuccilloMarco Romano
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