Helix unwinding in the effector region of elongation factor EF-Tu-GDP

Structure
G PolekhinaJ Nyborg

Abstract

Elongation factor Tu (EF-Tu) in its GTP conformation is a carrier of aminoacylated tRNAs (aa-tRNAs) to the ribosomal A site during protein biosynthesis. The ribosome triggers GTP hydrolysis, resulting in the dissociation of EF-Tu-GDP from the ribosome. The affinity of EF-Tu for other molecules involved in this process, some of which are unknown, is regulated by two regions (Switch I and Switch II) that have different conformations in the GTP and GDP forms. The structure of the GDP form of EF-Tu is known only as a trypsin-modified fragment, which lacks the Switch I, or effector, domain. The aim of this work was to establish the overall structure of intact EF-Tu-GDP, in particular the structure of the effector domain. The crystal structures of intact EF-Tu-GDP from Thermus aquaticus and Escherichia coli have been determined at resolutions of 2.7 A and 3.8 A, respectively. The structures confirm the domain orientation previously found in the structure of partially trypsin-digested EF-Tu-GDP. The structures of the effector region in T. aquaticus and E. coli EF-Tu-GDP are very similar. The C-terminal part of the effector region of EF-Tu-GDP is a beta hairpin; in EF-Tu-GTP, this region forms an alpha helix. This conformational change...Continue Reading

References

Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R C Thompson, P J Stone
Oct 5, 1976·Journal of Molecular Biology·W H GastA Wittinghofer
Jan 1, 1975·Biochimie·J Ninio
Nov 1, 1992·Acta Crystallographica. Section A, Foundations of Crystallography·D E Tronrud
Feb 5, 1992·Journal of Molecular Biology·M Kjeldgaard, J Nyborg
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J J Hopfield
Nov 1, 1970·Archives of Biochemistry and Biophysics·D L Miller, H Weissbach
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Jul 1, 1980·European Journal of Biochemistry·A WittinghoferR Leberman
Dec 1, 1981·European Journal of Biochemistry·K GulewiczM Sprinzl
Apr 15, 1981·Journal of Molecular Biology·C ThallerJ N Jansonius
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4

❮ Previous
Next ❯

Citations

Dec 10, 1999·Folia Microbiologica·B KraalC Kleanthous
Mar 5, 2005·Cellular and Molecular Life Sciences : CMLS·C R McCuddenF S Willard
Dec 15, 2010·European Biophysics Journal : EBJ·Katarzyna KulczyckaJoanna Trylska
Dec 26, 2012·European Biophysics Journal : EBJ·Ping Xie
Mar 17, 2004·Journal of Molecular Biology·Mutsuko Kukimoto-NiinoShigeyuki Yokoyama
Mar 25, 2000·International Journal for Parasitology·S SatoR J Wilson
Feb 19, 2003·Journal of Molecular Biology·Jeffrey A ChaoJames R Williamson
Aug 26, 2000·Progress in Biophysics and Molecular Biology·S Al-KaradaghiA Liljas
Dec 5, 1998·Biochimica Et Biophysica Acta·I M Krab, A Parmeggiani
Jun 17, 1999·FEMS Microbiology Reviews·M V RodninaW Wintermeyer
Feb 1, 1997·Current Opinion in Structural Biology·B F Clark, J Nyborg
Jan 22, 1998·Current Opinion in Structural Biology·M Geyer, A Wittinghofer
Jan 22, 1998·Current Opinion in Structural Biology·S R Sprang
Aug 23, 2003·Trends in Biochemical Sciences·Gregers R AndersenJens Nyborg
Aug 4, 1999·Trends in Biochemical Sciences·J Cherfils, P Chardin
Dec 23, 2009·Quarterly Reviews of Biophysics·Xabier Agirrezabala, Joachim Frank
Mar 9, 2011·Biochemistry·Sravanti Vaidya, Jeanne A Hardy
Mar 14, 2012·Biochemistry·Darius KavaliauskasCharlotte R Knudsen
Jun 17, 1998·Nature Structural Biology·E NogalesJ Löwe
Aug 1, 1997·Nature Structural Biology·Y WangP B Sigler
Nov 24, 1999·The Biochemical Journal·J HeierhorstB E Kemp
Oct 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Vasili HauryliukAlexander A Makarov
Jan 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth VillaJoachim Frank
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·M V Rodnina, W Wintermeyer
Oct 11, 2007·The Journal of Biological Chemistry·Kirill B GromadskiMarina V Rodnina
May 7, 2009·The Journal of Biological Chemistry·Alexander S Spirin
Feb 17, 2000·The EMBO Journal·P NissenJ Nyborg
May 3, 2000·The EMBO Journal·J TomsicC O Gualerzi
Jul 3, 2002·The EMBO Journal·Mikel ValleJoachim Frank
Sep 24, 1999·Protein Science : a Publication of the Protein Society·M YoungS Highsmith
Nov 25, 1997·European Journal of Biochemistry·T RattenborgC R Knudsen
Dec 24, 2004·Antimicrobial Agents and Chemotherapy·Maithri M K JayasekeraKaren Joy Shaw
Sep 17, 2008·Molecular and Cellular Biology·Pankaj V AloneThomas E Dever
Jun 17, 2011·Annual Review of Biochemistry·Alfred Wittinghofer, Ingrid R Vetter
Jan 1, 1997·Annual Review of Biochemistry·S R Sprang
Jul 19, 2003·RNA·Stefano MarziJ Stephen Lodmell

❮ Previous
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