PMID: 21500568Apr 20, 2011

Hemagglutinin gene of influenza A (H3N2) virus from human samples in the 2009 influenza epidemic in Chengdu

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
Dong-ling LongHeng-chuan Liu

Abstract

To study the antigenic and genetic characteristics of influenza A (H3N2) virus in the 2009 influenza epidemics in Chengdu. The influenza virus strains were isolated with MDCK cells from 4869 samples taken from the sentinel surveillance in 2009 in Chengdu. Hemagglutination inhibition (HI) and RT-PCR reaction tests were performed to guide the extraction of viral RNA from the culture fluid of the influenza A (H3N2) virus. The hemagglutinin gene was obtained by RT-PCR and sequenced. The separation rates of swine influenza H1N1, H3N2, H1N1, and B were 25.2%, 7.2%, 4.5%, and 1.5% respectively. The epidemic peaked in summer and autumn. Four amino acids changed in A, B, and D antigenic and receptor binding sites: site160N>K, site174K>R/N, site189K>Q, site277R>Q. Glycosylation sites were inserted to sitel60 or absent at site181 in some isolated strains. Swine influenza H1N1 viruses dominated the 2009 Chengdu epidemic, with H3N2, H1N1, and B strains coexisting. The influenza A(H3N2) viruses had gene variations due to antigenic drift.

Related Concepts

H1 hemagglutinin, human 1918 influenza virus
Influenza
RNA, Viral
Variation (Genetics)
Sequence Determinations, RNA
Hemagglutinin Glycoproteins, Influenza Virus
Influenza A Virus, H3N2 Subtype
Antigens
Genes
Glutamine

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