Hematocrit-independent recovery of immunosuppressants from DBS using heated flow-through desorption

Bioanalysis
Christel Maria HempenJan Albert Ooms

Abstract

We applied our concept for automated flow-through desorption of DBS to investigate the effect of desorption temperature on recovery. For this purpose, a method has been developed for the determination of four immunosuppressants in DBS. We compared recoveries of four immunosuppressants for measurements with and without temperature-enhanced desorption at different hematocrit (Ht) levels. Temperature-enhanced desorption increased recovery substantially for tacrolimus, sirolimus and everolimus at all Ht values and for cyclosporine At high Ht. In addition, recovery became largely independent from Ht variations. Under the optimized conditions, a brief validation using spiked blood samples showed that the method complies with acceptance criteria for quantitative bioanalysis. This method enables a quantitative analysis of immunosuppressants in DBS independent from the Ht.

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Citations

May 13, 2017·Toxicon : Official Journal of the International Society on Toxinology·Rebekah E WhartonRudolph C Johnson
Nov 22, 2018·Journal of Analytical Toxicology·Craig SeymourRudolph C Johnson
Sep 25, 2014·Mass Spectrometry Reviews·Michel WagnerGérard Hopfgartner
Nov 29, 2017·Analytical Methods : Advancing Methods and Applications·Rebecca L ShanerRudolph C Johnson

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