Hematopoietic cells expressing the peripheral cannabinoid receptor migrate in response to the endocannabinoid 2-arachidonoylglycerol.

Blood
Meritxell Alberich JordàRuud Delwel

Abstract

Cb2 is a novel protooncogene encoding the peripheral cannabinoid receptor. Previous studies demonstrated that 2 distinct noncoding first exons exist: exon-1A and exon-1B, which both splice to protein-coding exon-2. We demonstrate that in retrovirally induced murine myeloid leukemia cells with proviral insertion in Cb2, exon-1B/exon-2 Cb2 messenger RNA levels have been increased, resulting in high receptor numbers. In myeloid leukemia cells without virus insertion in this locus, low levels of only exon-1A/exon-2 Cb2 transcripts were present and receptors could not be detected. To elucidate the function of Cb2 in myeloid leukemia cells, a set of in vitro experiments was carried out using 32D/G-CSF-R (granulocyte colony-stimulating factor receptor) cells transfected with exon-1B/exon-2 Cb2 complementary DNA and a myeloid cell line carrying a virus insertion in Cb2 (ie, NFS 78). We demonstrate that a major function of the Cb2 receptor is stimulation of migration as determined in a transwell assay. Exposure of Cb2-expressing cells to different cannabinoids showed that the true ligand for Cb2 is 2-arachidonoylglycerol (2-AG), which may act as chemoattractant and as a chemokinetic agent. Furthermore, we observed a significant synergis...Continue Reading

References

Oct 1, 1976·The Journal of Allergy and Clinical Immunology·G S RachelefskyE R Stiehm
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·L F AllenS Cotecchia
Jan 1, 1991·Advances in Experimental Medicine and Biology·G G Nahas, E F Osserman
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K L HolmesJ N Ihle
Jun 1, 1986·Journal of Leukocyte Biology·M Lopez-CeperoH Friedman
Jan 1, 1986·International Journal of Immunopharmacology·S C SpecterH Friedman
Jan 1, 1987·Journal of Toxicology and Environmental Health·T W KleinH Friedman
May 1, 1988·Journal of Neurochemistry·K Herrick-Davis, M Titeler
Oct 4, 1995·Biochemical and Biophysical Research Communications·T SugiuraK Waku
Jun 1, 1995·Endocrine Reviews·N DhanasekaranG L Johnson
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·L FacciA Leon
Feb 1, 1995·Molecular and Cellular Biology·I WhiteheadR Kay
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·T NagasawaT Kishimoto
Feb 1, 1994·Trends in Pharmacological Sciences·W A Devane
May 15, 1993·European Journal of Biochemistry·M BouaboulaP Casellas
May 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G R Post, J H Brown
Aug 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M Kozak
Dec 1, 1996·Endocrine Reviews·T van BiesenR J Lefkowitz
Sep 1, 1996·The Journal of Experimental Medicine·C C BleulT A Springer
Nov 5, 1997·The Journal of Biological Chemistry·X SanchezJ Navarro
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·E R Neptune, H R Bourne
Jul 17, 1998·Advances in Experimental Medicine and Biology·T W KleinH Friedman
Oct 21, 1998·Oncogene·S Offermanns, M I Simon
Oct 21, 1998·Oncogene·N DhanasekaranD Onesime
Jan 19, 1999·Annual Review of Cell and Developmental Biology·N K Gray, M Wickens
Jan 28, 1999·Molecular and Cellular Endocrinology·E R Simpson

❮ Previous
Next ❯

Citations

Jun 1, 2013·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Rodrigo GuabirabaMauro M Teixeira
Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Thomas W Klein, Guy A Cabral
Feb 6, 2008·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Erinn S RabornGuy A Cabral
Sep 17, 2009·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Tracy A SherwoodThomas W Klein
Jan 21, 2009·Expert Reviews in Molecular Medicine·Guy A Cabral, LaToya Griffin-Thomas
Apr 7, 2011·Cell Research·Nariman A B BalengaMaria Waldhoer
Mar 3, 2009·Nature Immunology·João P PereiraJason G Cyster
Nov 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jungwoo LeeBiju Parekkadan
Sep 9, 2006·Current Opinion in Lipidology·Sabine Steffens, Francois Mach
Jan 22, 2010·The Journal of Pharmacology and Experimental Therapeutics·Erinn S Raborn, Guy A Cabral
Jun 27, 2006·The AAPS Journal·Ken Mackie, Nephi Stella
Jul 2, 2011·Biological & Pharmaceutical Bulletin·Takashi TanikawaYasuyuki Imai
Jul 18, 2006·Biochemical and Biophysical Research Communications·Masashi KohnoMasaki Yasukawa
Sep 20, 2006·Expert Opinion on Therapeutic Targets·Charles A LunnLoretta Bober
Dec 1, 2006·Expert Opinion on Drug Discovery·Terry Kenakin
Feb 16, 2010·Immunobiology·Radu Tanasescu, Cris S Constantinescu
Aug 30, 2008·Neuropharmacology·Nephi Stella
Aug 12, 2008·Neuropharmacology·Jason R ClapperDaniele Piomelli
Sep 10, 2008·European Journal of Pharmacology·Deborah PatinkinRaphael Mechoulam
Oct 20, 2007·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Regina A Mangieri, Daniele Piomelli
Feb 9, 2005·British Journal of Pharmacology·Angelo VaccaniDaniela Parolaro
Feb 6, 2004·British Journal of Pharmacology·Lisa Walter, Nephi Stella
Oct 2, 2007·British Journal of Pharmacology·C A LunnL Bober
Aug 21, 2007·British Journal of Pharmacology·M D Randall
Aug 23, 2007·Chemistry & Biodiversity·Giulio G Muccioli
Feb 28, 2006·British Journal of Pharmacology·Maurizio BifulcoPatrizia Gazzerro
Jul 24, 2008·Journal of Thrombosis and Haemostasis : JTH·S BaldassarriF Sinigaglia
Nov 6, 2007·British Journal of Pharmacology·A M Miller, N Stella
Oct 16, 2015·Journal of Cellular Biochemistry·Maria Grazia Signorello, Giuliana Leoncini
Jul 19, 2005·Journal of Neuroimmunology·J Ludovic Croxford, Takashi Yamamura
Oct 1, 2013·Progress in Lipid Research·Ismael Galve-RoperhMauro Maccarrone

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.