Hematopoietic recovery in cancer patients after transplantation of autologous peripheral blood CD34+ cells or unmanipulated peripheral blood stem and progenitor cells

Transfusion
Y BeguinG Fillet

Abstract

A study of CD34+ cell selection and transplantation was carried out with particular emphasis on characteristics of short- and long-term hematopoietic recovery. Peripheral blood stem and progenitor cells (PBPCs) were collected from 32 patients, and 17 CD34+ cell-selection procedures were carried out in 15 of the 32. One patient in whom two procedures failed to provide 1 x 10(6) CD34+ cells per kg was excluded from further analysis. After conditioning, patients received CD34+ cells (n = 10, CD34 group) or unmanipulated (n = 17, PBPC group) PBPCs containing equivalent amounts of CD34+ cells or progenitors. The yield of CD34+ cells was 53 percent (18-100) with a purity of 63 percent (49-82). The CD34+ fraction contained 66 percent of colony-forming units--granulocyte-macrophage (CFU-GM) and 58 percent of CFU of mixed lineages, but only 33 percent of burst-forming units-erythroid (BFU-E) (p < 0.05). Early recovery of neutrophils and reticulocytes was identical in the two groups, although a slight delay in platelet recovery may be seen with CD34+ cell selection. Late hematopoietic reconstitution, up to 1.5 years after transplant, was also similar. The two groups were thus combined for analyses of dose effects. A dose of 40 x 10(4) CF...Continue Reading

Citations

Jul 6, 2000·Medical and Pediatric Oncology·J KanoldF Deméocq
Aug 28, 2003·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Tommaso CaravitaGiancarlo Isacchi
Oct 17, 2012·Blood Reviews·Ibraheem H Motabi, John F DiPersio
Jan 13, 2006·European Journal of Haematology·Pascale FrèreYves Beguin
Aug 30, 2008·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Iskra PusicJohn F DiPersio
Oct 22, 2003·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Nathan GunnCharles Linker
Sep 25, 2007·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Javier Bolaños-MeadeGeorgia B Vogelsang
Jun 7, 2008·Veterinary Immunology and Immunopathology·Petra ZelnickovaMartin Faldyna
Mar 15, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·S SienaC Carlo-Stella

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