Abstract
Mesenchymal stem cells (MSCs) are heterogeneous population of cells with great potential for regenerative medicine. MSCs are relatively easy to expand in a cell culture, however determination of their concentration in harvested tissue is more complex and is not implemented as routine procedure. To identify MSCs collected from bone marrow we have used two combinations of cell markers (CD45-/CD73+/CD90+/CD105+ and CD45-/CD271+) and fibroblast colony-forming unit (CFU-F) assay. Further, in donors of various ages, mesenchymal stem cell concentration was compared with the result of CFU-F assay and with hematopoietic stem cell concentration, determined by a standardized flow cytometric assay. A positive correlation of MSC populations to the CFU-F numbers is observed, the population of the CD45-/CD271+ cells correlates better with CFU-F numbers than the population of the CD45-/CD73+/CD90+/CD105+ cells. The relationship between the hematopoietic CD45dim/CD34+ cell concentration and mesenchymal CFU-Fs or CD45-/CD271+ cells shows a positive linear regression. An age-related quantitative reduction of hematopoietic CD45dim/CD34+, mesenchymal CD45-/CD73+/CD90+/CD105+ and CD45-/CD271+ stem cells, and CFU-F numbers were noted. Additionally, s...Continue Reading
References
Jan 19, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·O N KoçH M Lazarus
Jul 24, 2002·Experimental Hematology·Nadia QuiriciGiorgio Lambertenghi Deliliers
Aug 23, 2006·Cytotherapy·M DominiciEm Horwitz
Mar 31, 2007·Annals of the New York Academy of Sciences·Hans-Jörg BühringWichard Vogel
Jul 20, 2007·Blood·Lynne M BallWillem E Fibbe
Feb 5, 2008·Mechanisms of Ageing and Development·A StolzingA Scutt
Jun 23, 2009·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Partow KebriaeiJoseph Uberti
Nov 18, 2009·Acta Orthopaedica·Eelco M FennemaJan de Boer
Feb 25, 2010·Haematologica·Selim KuçiPeter Bader
May 12, 2010·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Vinod K PrasadJoanne Kurtzberg
Oct 26, 2010·Aging Cell·Ji Min YuBruce A Bunnell
Mar 23, 2011·Aging Cell·Klaudia KurandaMichele Goodhardt
Oct 14, 2011·Haematologica·Marijke W MaijenburgCarlijn Voermans
Nov 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Wendy W PangIrving L Weissman
Jan 25, 2012·Cytotherapy·Richard CuthbertElena Jones
Jul 12, 2013·Annals of Hematology·Yamei WuZhongchao Han
Sep 13, 2013·Frontiers in Immunology·Amy M DimarinoTracey L Bonfield
Feb 7, 2014·Pediatric Hematology and Oncology·Michael M SchündelnBernhard Kremens
Dec 30, 2014·PloS One·Olivia S BeaneEric M Darling
Mar 13, 2015·Blood·Philip E Boulais, Paul S Frenette
Mar 13, 2015·Blood·Connie J Eaves
Aug 25, 2015·The Journal of Dermatology·Hirohiko AkamatsuKayoko Matsunaga
May 18, 2016·Transfusion·Petra Konecnik KresnikElvira Malicev
May 22, 2016·Clinical Reviews in Allergy & Immunology·Alexandre T J MariaPhilippe Guilpain
Citations
Oct 10, 2019·The Journal of Bone and Joint Surgery. American Volume·Jehan J El-JawhariPeter V Giannoudis
Dec 5, 2019·The Journal of Bone and Joint Surgery. American Volume·Jehan J El-JawhariPeter V Giannoudis
Jan 3, 2021·Osteoarthritis and Cartilage·H Madry, E B Hunziker
Oct 2, 2020·Journal of Clinical Orthopaedics and Trauma·José Fábio Santos Duarte LanaJoseph Purita
Dec 11, 2020·Stem Cells International·Tiago O RibeiroVitor Fortuna
Jan 26, 2021·Frontiers in Cell and Developmental Biology·Amandine GirousseCoralie Sengenès
Jan 16, 2021·Current Osteoporosis Reports·Sana AnsariSandra Hofmann
Mar 27, 2021·Transfusion·Wolfgang RennertCorina Gonzalez
Apr 4, 2021·International Journal of Molecular Sciences·José Fábio LanaMarco Antonio Percope de Andrade
Jun 3, 2021·Bioengineering·Jehan J El-JawhariPeter V Giannoudis