Hematopoietic stem cell and mesenchymal stem cell population size in bone marrow samples depends on patient's age and harvesting technique

Cytotechnology
Katja ReboljElvira Maličev

Abstract

Mesenchymal stem cells (MSCs) are heterogeneous population of cells with great potential for regenerative medicine. MSCs are relatively easy to expand in a cell culture, however determination of their concentration in harvested tissue is more complex and is not implemented as routine procedure. To identify MSCs collected from bone marrow we have used two combinations of cell markers (CD45-/CD73+/CD90+/CD105+ and CD45-/CD271+) and fibroblast colony-forming unit (CFU-F) assay. Further, in donors of various ages, mesenchymal stem cell concentration was compared with the result of CFU-F assay and with hematopoietic stem cell concentration, determined by a standardized flow cytometric assay. A positive correlation of MSC populations to the CFU-F numbers is observed, the population of the CD45-/CD271+ cells correlates better with CFU-F numbers than the population of the CD45-/CD73+/CD90+/CD105+ cells. The relationship between the hematopoietic CD45dim/CD34+ cell concentration and mesenchymal CFU-Fs or CD45-/CD271+ cells shows a positive linear regression. An age-related quantitative reduction of hematopoietic CD45dim/CD34+, mesenchymal CD45-/CD73+/CD90+/CD105+ and CD45-/CD271+ stem cells, and CFU-F numbers were noted. Additionally, s...Continue Reading

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Citations

Oct 10, 2019·The Journal of Bone and Joint Surgery. American Volume·Jehan J El-JawhariPeter V Giannoudis
Dec 5, 2019·The Journal of Bone and Joint Surgery. American Volume·Jehan J El-JawhariPeter V Giannoudis
Jan 3, 2021·Osteoarthritis and Cartilage·H Madry, E B Hunziker
Oct 2, 2020·Journal of Clinical Orthopaedics and Trauma·José Fábio Santos Duarte LanaJoseph Purita
Jan 26, 2021·Frontiers in Cell and Developmental Biology·Amandine GirousseCoralie Sengenès
Jan 16, 2021·Current Osteoporosis Reports·Sana AnsariSandra Hofmann
Apr 4, 2021·International Journal of Molecular Sciences·José Fábio LanaMarco Antonio Percope de Andrade

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