Hematopoietic stem cells provide repair functions after laser-induced Bruch's membrane rupture model of choroidal neovascularization

The American Journal of Pathology
Tailoi Chan-LingMaria B Grant

Abstract

Vascular repair by adult hematopoietic stem cells (HSCs) is well-appreciated because these cells are known for their plasticity. We have shown that adult HSCs differentiate into endothelial cells and participate in both retinal and choroidal neovascularization. We asked whether HSCs participated in the wounding response by forming astrocytes, retinal pigment epithelia (RPE), macrophages, and pericytes. Lethally irradiated C57BL6/J mice were reconstituted with HSCs from mice homozygous for green fluorescent protein (GFP) and then subjected to laser-induced rupture of Bruch's membrane. After immunohistochemical examination of ocular tissue, GFP(+) astrocytes were observed concentrated along the edge of the laser wound, where they and mural cells closely ensheathed the neovasculature. GFP(+) vascular endothelial cells and macrophages/microglia were also evident. Large irregularly shaped GFP(+) RPE cells constituted approximately 93% of RPE cells adjacent to the edge of the denuded RPE area. In regions farther away from the wound, GFP(+) RPE cells were integrated among the GFP(-) host RPE. Thus, postnatal HSCs can differentiate into cells expressing markers specific to astrocytes, macrophages/microglia, mural cells, or RPE. These s...Continue Reading

References

Aug 15, 1989·The Journal of Comparative Neurology·T L LingJ Stone
Jul 1, 1988·Science·G J SpangrudeI L Weissman
Apr 1, 1987·Journal of Neurocytology·W J Streit, G W Kreutzberg
Feb 1, 1971·The Journal of Experimental Medicine·J FolkmanG Williams
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D E MaddoxI J Goldstein
Jan 1, 1997·Microscopy Research and Technique·T Chan-Ling
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·Q MaT A Springer
Apr 2, 1999·Science·M F PittengerD R Marshak
May 15, 1999·Science·B E PetersenJ P Goff
Aug 23, 2000·The Journal of Clinical Investigation·G C SchattemanB A Christy
Apr 5, 2002·Nature·Qi-Long YingAustin G Smith
Jun 6, 2002·Journal of Cellular Biochemistry. Supplement·Kathyjo A JacksonMargaret A Goodell
May 29, 2003·Leukemia & Lymphoma·Koichi HattoriShahin Rafii
Jun 12, 2003·Annals of the New York Academy of Sciences·Donald Orlic
Aug 2, 2003·Journal of Vascular Research·E Sivan-LoukianovaG C Schatteman
Oct 14, 2003·Nature·Manuel Alvarez-DoladoArturo Alvarez-Buylla
Oct 28, 2003·Investigative Ophthalmology & Visual Science·Nilanjana SenguptaMaria B Grant
Oct 28, 2003·Investigative Ophthalmology & Visual Science·Diego G Espinosa-HeidmannScott W Cousins
Nov 26, 2003·Investigative Ophthalmology & Visual Science·Volker EnzmannHenry J Kaplan
Feb 26, 2004·International Journal of Hematology·Michael S RutenbergNaohiro Terada
Apr 27, 2004·Trends in Molecular Medicine·Nemanja RodićNaohiro Terada
May 27, 2004·Investigative Ophthalmology & Visual Science·Tailoi Chan-LingGerard A Lutty
Jul 28, 2004·Investigative Ophthalmology & Visual Science·Suzanne Hughes, Tailoi Chan-Ling
Jul 28, 2004·Stem Cells·Joanna E GroveDiane S Krause
Oct 29, 2004·The American Journal of Pathology·Xenia BorueDiane S Krause
May 17, 2005·Neuroscience·S BonillaS Martínez
Jun 17, 2005·Annals of the New York Academy of Sciences·Koichi Akashi
Jun 17, 2005·Annals of the New York Academy of Sciences·Peter J QuesenberryDeborah Greer
Aug 2, 2005·Neuroreport·Keizo MinaminoSusumu Ikehara

❮ Previous
Next ❯

Citations

May 26, 2011·Current Diabetes Reports·Lynn C ShawMaria B Grant
Jul 9, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Nilanjana SenguptaJeffrey R Harris
Aug 1, 2006·Photomedicine and Laser Surgery
May 13, 2011·Stem Cells and Development·Tajinder SinghAkshay Anand
Apr 27, 2013·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Mohd Imtiaz NawazMohammad Shamsul Ola
May 14, 2009·The American Journal of Pathology·Sandrine JolyCharlotte E Remé
Feb 28, 2015·Biological Research·Gustavo OrtizJuan E Gallo
Aug 10, 2007·Expert Opinion on Investigational Drugs·Cristina Hernández, Rafael Simó
Apr 19, 2012·Molecular Aspects of Medicine·Stuart G Jarrett, Michael E Boulton
Mar 24, 2017·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Gloriane SchnabolkBärbel Rohrer
Feb 17, 2017·Molecular Therapy : the Journal of the American Society of Gene Therapy·Xiaoping QiMichael E Boulton
Nov 30, 2006·Journal of Cellular Physiology·Peter A Campochiaro
May 8, 2008·The Journal of Comparative Neurology·Brian Tiong Gee TanRunsheng Ruan
May 25, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Raquel Lima e SilvaPeter A Campochiaro
Jul 23, 2019·European Journal of Ophthalmology·Dario Pasquale MuccioloAnna Maria Gori
Dec 19, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zerina LokmicGeraldine M Mitchell
Dec 19, 2019·Frontiers in Pharmacology·Natalie Jia Ying YeoChristine Cheung
Oct 21, 2009·Current Eye Research·Anna MachalińskaBogusław Machaliński
May 29, 2007·Progress in Retinal and Eye Research·Susanne BinderCarl Glittenberg
Oct 26, 2007·Seminars in Cell & Developmental Biology·Graziella PellegriniYvan Arsenijevic
Dec 15, 2007·Experimental Eye Research·Rūta Motiejūnaite, Andrius Kazlauskas

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.