Hematoporphyrin monomethyl ether-mediated photodynamic effects on THP-1 cell-derived macrophages

Journal of Photochemistry and Photobiology. B, Biology
Jiali ChengWenwu Cao

Abstract

Photodynamic therapy (PDT) has been shown to attenuate atherosclerotic plaque progression and decrease macrophage-infiltration. The effectiveness of PDT depends strongly on the type of photosensitizers. Hematoporphyrin monomethyl ether (HMME) is a promising second-generation porphyrin-related photosensitizer for PDT. This study is designed to characterize effects of HMME-based PDT on THP-1 cell-derived macrophages and define the cell-death pathway. HMME was identified to accumulate in the macrophages by fluorescence microscopy and confocal scanning laser microscope. Our data demonstrated that the intensity of laser-induced HMME fluorescence in macrophages steadily increased with the increasing incubation concentration of HMME. The survival rate of macrophages determined by MTT assay decreased with the increasing HMME concentration and irradiation time. HMME-based PDT induced macrophage apoptosis via caspase-9 and caspase-3 activation pathway detected by caspase fluorescent assay kit and flow cytometer. The PDT increased the number of apoptotic macrophages by 14-fold at 12 h post irradiation by 9 J/cm(2) 635 nm diode laser. These results imply that photodynamic therapy with HMME may therefore be a useful clinical treatment for u...Continue Reading

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Citations

Mar 20, 2013·Cancer Biotherapy & Radiopharmaceuticals·Xiaomin SuQuanhong Liu
May 7, 2014·Lasers in Medical Science·Lifeng LiuJiwu Han
Dec 3, 2014·Photodiagnosis and Photodynamic Therapy·Xiaoming ZhangShaoshan Hu
May 19, 2012·Photodiagnosis and Photodynamic Therapy·Yu PuZhengwei Yu
Mar 5, 2016·Future Microbiology·Chengcheng LiuJiru Xu
Oct 17, 2014·FEMS Microbiology Letters·Deshu ZhuangQi Zhou
Nov 7, 2016·Journal of Photochemistry and Photobiology. B, Biology·Ping-Yong LiaoZhi-Long Chen
Mar 7, 2017·Physical Chemistry Chemical Physics : PCCP·Huimin ZhaoChengshan Guo
Jul 26, 2017·Journal of Biomaterials Applications·Weiwei JinYu Hu
Oct 18, 2018·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Wei MaWenwu Cao
Jan 15, 2020·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Xiaomei CuiXiaodong Chen
Aug 8, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Yi ZhangXiaojia Huang

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