PMID: 7524095Oct 25, 1994Paper

Heme coordination of NO in NO synthase

Proceedings of the National Academy of Sciences of the United States of America
J WangD J Stuehr

Abstract

A current question in nitric oxide (NO) biology is whether NO can act as a feedback inhibitor of NO synthase (NOS). We have approached this problem by examining the interaction of NO with neuronal NOS by optical absorption and resonance Raman scattering spectroscopies. Under an inert atmosphere NO coordinated to the heme iron in both the oxidized and reduced forms of NOS. The Soret and visible optical absorption transitions are detected at 436 and at 567 nm, respectively, in the Fe(2+)-NO heme complex and at 440 nm and at 549 and 580 nm, respectively, in the Fe(3+)-NO heme complex. In the resonance Raman spectrum of the ferrous complex the Fe-NO stretching mode is located at 549 cm-1 in the presence of L-arginine and at 536 cm-1 in the absence of L-arginine, whereas in the ferric enzyme the mode is located at 540 cm-1 (in the absence of L-arginine). The interaction between bound L-arginine and the NO indicates that L-arginine binds directly over the heme just as do the substrates in cytochrome P-450s. In the absence of L-arginine, NO readily oxidized the ferrous heme iron. The oxidation was prevented by the presence of bound L-arginine and enabled NOS to form a stable ferrous NO complex. Under oxygen-limited conditions, NO gene...Continue Reading

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Citations

Sep 1, 1997·Journal of Cellular Physiology·T VolkW J Kox
Apr 11, 2001·Journal of Inorganic Biochemistry·S Adak, D J Stuehr
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·J WangD L Rousseau
Feb 12, 2011·The Journal of Biological Chemistry·Albane BrunelJérôme Santolini
Nov 27, 2009·Antioxidants & Redox Signaling·Mayumi KajimuraMakoto Suematsu
Jul 15, 1997·European Journal of Biochemistry·W K McCoubreyM D Maines
Jan 1, 1997·Annual Review of Pharmacology and Toxicology·D J Stuehr
Jan 1, 1997·Annual Review of Pharmacology and Toxicology·M D Maines
Aug 1, 1997·American Journal of Respiratory and Critical Care Medicine·X CombesP Hervé
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Semira GalijasevicHusam M Abu-Soud
Jun 29, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Huiying LiThomas L Poulos
Mar 16, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Douglas P Linder, Kenton R Rodgers
Oct 22, 2009·The FEBS Journal·John C Salerno, Dipak K Ghosh
Nov 13, 2007·Biochimica Et Biophysica Acta·Marten H Vos
Apr 7, 2004·The Journal of Biological Chemistry·David LiDenis L Rousseau
Oct 30, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Jean A WyerSteen Brøndsted Nielsen
Sep 10, 2014·Chembiochem : a European Journal of Chemical Biology·Toni Kühl, Diana Imhof
Dec 18, 2018·Applied Spectroscopy·Carolin HartmannNatalia P Ivleva
Jan 20, 2006·The Journal of Biological Chemistry·David LiSyun-Ru Yeh
Aug 24, 1999·The Journal of Biological Chemistry·M NégrerieJ L Martin
Apr 25, 2002·The Journal of Biological Chemistry·Eftychia PinakoulakiConstantinos Varotsis
Nov 9, 2000·The Journal of Biological Chemistry·J S ScheeleD Magde
Apr 5, 2000·The Journal of Biological Chemistry·S AdakD J Stuehr
Sep 12, 2012·Angewandte Chemie·Jean Ann Wyer, Steen Brøndsted Nielsen

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