Heme destruction, the main molecular event during the peroxide-mediated inactivation of chloroperoxidase from Caldariomyces fumago

Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry
Marcela AyalaRafael Vazquez-Duhalt


Heme peroxidases are subject to a mechanism-based oxidative inactivation. During the catalytic cycle, the heme group is activated to form highly oxidizing species, which may extract electrons from the protein itself. In this work, we analyze changes in residues prone to oxidation owing to their low redox potential during the peroxide-mediated inactivation of chloroperoxidase from Caldariomyces fumago under peroxidasic catalytic conditions. Surprisingly, we found only minor changes in the amino acid content of the fully inactivated enzyme. Our results show that tyrosine residues are not oxidized, whereas all tryptophan residues are partially oxidized in the inactive protein. The data suggest that the main process leading to enzyme inactivation is heme destruction. The molecular characterization of the peroxide-mediated inactivation process could provide specific targets for the protein engineering of this versatile peroxidase.


Dec 10, 1999·The Journal of Biological Chemistry·O M LardinoisPaul R Ortiz de Montellano
Mar 29, 2000·Biochemical and Biophysical Research Communications·O M Lardinois, Paul R Ortiz de Montellano
Mar 14, 2001·Biochimica Et Biophysica Acta·C L Hawkins, M J Davies
May 19, 2001·European Journal of Biochemistry·A N HinerJ N Rodríguez-López
Jun 19, 2001·Biochimica Et Biophysica Acta·Kelath Murali Manoj, Lowell P Hager
Jan 5, 2002·The Journal of Biological Chemistry·Christophe ColasPaul R Ortiz de Montellano
May 29, 2002·Chemistry & Biology·Brenda ValderramaRafael Vazquez-Duhalt
Dec 27, 2005·Archives of Biochemistry and Biophysics·Liusheng Huang, Paul R Ortiz de Montellano
Jan 21, 2006·Biotechnology and Bioengineering·Jin-Byung Park, Douglas S Clark
Apr 22, 2006·Applied Microbiology and Biotechnology·Martin Hofrichter, René Ullrich
May 25, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Brenda ValderramaRafael Vazquez-Duhalt
Apr 20, 2007·Biochemical and Biophysical Research Communications·Marcela AyalaRafael Vazquez-Duhalt
May 12, 2007·Chembiochem : a European Journal of Chemical Biology·Carl Elovson GreyPatrick Adlercreutz
Jan 29, 2008·Biochemistry·Kelath Murali Manoj, Lowell P Hager


Jun 12, 2013·Applied Microbiology and Biotechnology·Milja PešićGregorio Alvaro
Mar 2, 2016·Archives of Biochemistry and Biophysics·Rui ZhangXiaotang Wang
Jul 3, 2013·Vector Borne and Zoonotic Diseases·Ljubo BarbicGiovanni Savini
Oct 17, 2019·Enzyme and Microbial Technology·Flor Sánchez-AlejandroRafael Vazquez-Duhalt

Related Concepts

Chloride Peroxidase
High Pressure Liquid Chromatography Procedure
Circular Dichroism, Vibrational
Enzyme Activation
Protein Engineering
Chloride Peroxidase

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, as well as fevers and skin rashes. Here is the latest research on this disease.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.


Trichotillomania is an impulse control disorder characterized by the recurrent pulling of one's hair resulting in noticeable hair loss. Here are the latest discoveries in this field.

DDX3X Syndrome

DDX3X syndrome is caused by a spontaneous mutation at conception that primarily affects females due to its location on the X-chromosome. DDX3X syndrome has been linked to intellectual disabilities, seizures, autism, low muscle tone, brain abnormalities, and slower physical developments. Here is the latest research.

Related Papers

Biotechnology and Bioengineering
Jin-Byung Park, Douglas S Clark
Biochemical and Biophysical Research Communications
J GeigertS L Neidleman
Kelath Murali Manoj, Lowell P Hager
© 2021 Meta ULC. All rights reserved