Heme oxygenase-1 induction depletes heme and attenuates pulmonary artery relaxation and guanylate cyclase activation by nitric oxide

American Journal of Physiology. Heart and Circulatory Physiology
Christopher J MingoneMichael S Wolin

Abstract

This study examines in endothelium-denuded bovine pulmonary arteries the effects of increasing heme oxygenase-1 (HO-1) activity on relaxation and soluble guanylate cyclase (sGC) activation by nitric oxide (NO). A 24-h organ culture with 0.1 mM cobalt chloride (CoCl2) or 30 microM Co-protoporphyrin IX was developed as a method of increasing HO-1 expression. These treatments increased HO-1 expression and HO activity by approximately two- to fourfold and lowered heme levels by 40-45%. Induction of HO-1 was associated with an attenuation of pulmonary arterial relaxation to the NO-donor spermine-NONOate. The presence of a HO-1 inhibitor 30 microM chromium mesoporphyrin during the 24-h organ culture (but not acute treatment with this agent) reversed the attenuation of relaxation to NO seen in arteries co-cultured with agents that increased HO-1. Relaxation to isoproterenol, which is thought to be mediated through cAMP, was not altered in arteries with increased HO-1. Inducers of HO-1 did not appear to alter basal sGC activity in arterial homogenates or expression of the beta(1)-subunit of sGC. However, the increase in activity seen in the presence of 1 microM spermine-NONOate was attenuated in homogenates obtained from arteries with ...Continue Reading

References

Sep 24, 1990·European Journal of Biochemistry·J H LinN G Abraham
Jan 1, 1988·The International Journal of Biochemistry·N G AbrahamS Shibahara
Jun 1, 1981·European Journal of Biochemistry·R GerzerG Schultz
Sep 30, 1982·Biochemical and Biophysical Research Communications·R GerzerD L Garbers
Jun 29, 1983·Biochimica Et Biophysica Acta·P A Craven, F R DeRubertis
Dec 22, 1999·The American Journal of Physiology·S A GupteM S Wolin
Jul 27, 2001·Circulation·L ZhaoM R Wilkins
Nov 13, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·J S Naik, B R Walker
Sep 25, 2002·Canadian Journal of Physiology and Pharmacology·S HoseinK Nakatsu
Oct 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nader G AbrahamMichael S Wolin
Oct 17, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Maria RaidlBettina Grasl-Kraupp
Jan 4, 2003·Hypertension·Fruzsina K JohnsonRobert A Johnson
Mar 21, 2003·The American Journal of Cardiology·David HarrisonHelmut Drexler
Apr 15, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Christopher J MingoneMichael S Wolin
Oct 8, 2003·Current Pharmaceutical Design·Roberto MotterliniColin J Green
Feb 3, 2004·American Journal of Respiratory Cell and Molecular Biology·Paul M HassounKenneth D Bloch
Sep 4, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Sachin A GupteMichael S Wolin
Nov 6, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Federico J TeranFruzsina K Johnson
Apr 12, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Saadet TurksevenNader G Abraham
Nov 8, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Christopher J MingoneMichael S Wolin
Apr 8, 2006·Physiological Reviews·Stefan W RyterAugustine M K Choi
Sep 7, 2006·The Journal of Clinical Investigation·Johannes-Peter StaschHarald H H W Schmidt

❮ Previous
Next ❯

Citations

Aug 12, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Mansoor AhmadMichael S Wolin
Dec 3, 2013·Canadian Journal of Physiology and Pharmacology·Saadet Turkseven, Elif Ertuna
Jan 20, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Michael S Wolin
Oct 16, 2013·Journal of Basic and Clinical Physiology and Pharmacology·Saadet TurksevenMukadder Yasa
Jun 21, 2020·Scientific Reports·Vu Thao-Vi DaoHarald H H W Schmidt
Jan 29, 2021·The Journal of Biological Chemistry·Dennis J StuehrArnab Ghosh
Mar 27, 2018·Nitric Oxide : Biology and Chemistry·Rohan C ShahAdam C Straub

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Free Radical Biology & Medicine
Darius J R Lane, Alfons Lawen
Journal of Bioenergetics and Biomembranes
Javier Rodríguez-AlonsoMiguel Angel Medina
American Journal of Physiology. Heart and Circulatory Physiology
Mansoor AhmadMichael S Wolin
© 2021 Meta ULC. All rights reserved