Heme oxygenase-1 induction mediates chemoresistance of breast cancer cells to pharmorubicin by promoting autophagy via PI3K/Akt pathway

Journal of Cellular and Molecular Medicine
Lei PeiNa Zhang

Abstract

Concerns about breast cancer had become the most dangerous cancer to women over the world, more and more anti-cancer agents are developed to treat this malignancy. Pharmorubicin is a cytotoxic drug, widely used in the treatment of breast cancer, but its role is limited because of chemoresistance produced by cells. This study focused on exploring the influence of autophagy on the resistance of pharmorubicin in breast cancer cells. The cell survival of breast cancer cells was detected by MTT. The mRNA expression of heme oxygenase-1 (HO-1) was tested by qRT-PCR. The protein expression of HO-1, autophagic proteins (LC3-I,LC3-II and Beclin-1), PI3K and Akt was detected by Western blot. Cell autophagy was examined by Cyto-ID Autophagy Detection Kit. After being treated with pharmorubicin, the expression of HO-1 and autophagy related proteins was significantly enhanced, but the cell survival ratio in the two cell lines decreased. After autophagy was inhibited, HO-1 expression in two cells was down-regulated. When pharmorubicin-resistant cells were transfected with si-HO-1, the cell survival decreased and the protein expression of HO-1, autophagic proteins (LC3-II/LC3-I and Beclin-1) as well as autophagy were all down-regulated, while ...Continue Reading

References

Jul 24, 2012·Naunyn-Schmiedeberg's Archives of Pharmacology·Manxiang LiShaojun Li
Jul 1, 2014·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·R ZhouH Chen
Aug 1, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Meng-meng LvJian-hua Zhao
Dec 24, 2014·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Yaghoub SafdariSafar Farajnia
Nov 14, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Liao NingLv Hai-Tong
Apr 12, 2016·Cancer Biomarkers : Section a of Disease Markers·Xi GuWen-Hai Zhang
Jun 16, 2016·Biochemical and Biophysical Research Communications·Ji-Hoon ParkKyung-Soon Park
May 2, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jia-Teng ZhongZhi-Wei Feng
Jun 22, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yuehua LiXiaoping Wu

❮ Previous
Next ❯

Citations

May 8, 2019·The Kaohsiung Journal of Medical Sciences·Zi-Ming HuangJia-Li Tao
Sep 11, 2019·In Vitro Cellular & Developmental Biology. Animal·Yanyan WangJunbo Ge
Nov 11, 2019·Breast Cancer Research and Treatment·Natasha TraceyValerie G Brunton
May 28, 2020·Journal of Clinical Medicine·Giuseppe SferrazzoIgnazio Barbagallo
Sep 8, 2020·Journal of Zhejiang University. Science. B·Nan WenZheng-Gui Du
Oct 31, 2020·Oxidative Medicine and Cellular Longevity·Ying ZhangLihong Yin
Nov 4, 2020·Free Radical Biology & Medicine·Malgorzata KucinskaMarek Murias
Jun 3, 2021·Antioxidants·Mariapaola NittiAnna Lisa Furfaro

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
PCR
MDA

Software Mentioned

ImageJ
GraphPad Prism

Related Concepts

Related Feeds

Parkinson's Disease & Autophagy (MDS)

Autophagy leads to degradation of damaged proteins and organelles by the lysosome. Impaired autophagy has been implicated in several diseases. Here is the role of autophagy in Parkinson’s disease.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Autophagy & Metabolism

Autophagy preserves the health of cells and tissues by replacing outdated and damaged cellular components with fresh ones. In starvation, it provides an internal source of nutrients for energy generation and, thus, survival. A powerful promoter of metabolic homeostasis at both the cellular and whole-animal level, autophagy prevents degenerative diseases. It does have a downside, however--cancer cells exploit it to survive in nutrient-poor tumors.

Autophagy & Disease

Autophagy is an important cellular process for normal physiology and both elevated and decreased levels of autophagy are associated with disease. Here is the latest research.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.