Abstract
Concerns about breast cancer had become the most dangerous cancer to women over the world, more and more anti-cancer agents are developed to treat this malignancy. Pharmorubicin is a cytotoxic drug, widely used in the treatment of breast cancer, but its role is limited because of chemoresistance produced by cells. This study focused on exploring the influence of autophagy on the resistance of pharmorubicin in breast cancer cells. The cell survival of breast cancer cells was detected by MTT. The mRNA expression of heme oxygenase-1 (HO-1) was tested by qRT-PCR. The protein expression of HO-1, autophagic proteins (LC3-I,LC3-II and Beclin-1), PI3K and Akt was detected by Western blot. Cell autophagy was examined by Cyto-ID Autophagy Detection Kit. After being treated with pharmorubicin, the expression of HO-1 and autophagy related proteins was significantly enhanced, but the cell survival ratio in the two cell lines decreased. After autophagy was inhibited, HO-1 expression in two cells was down-regulated. When pharmorubicin-resistant cells were transfected with si-HO-1, the cell survival decreased and the protein expression of HO-1, autophagic proteins (LC3-II/LC3-I and Beclin-1) as well as autophagy were all down-regulated, while ...Continue Reading
References
Dec 17, 2011·Journal of Proteomics·Duthika M MallawaaratchyRichard I Christopherson
Jul 24, 2012·Naunyn-Schmiedeberg's Archives of Pharmacology·Manxiang LiShaojun Li
May 15, 2013·Autophagy·Tianliang LiXiangguo Liu
Jul 5, 2013·Molecular Neurobiology·Y C TengA M Y Lin
Sep 11, 2013·Cell & Bioscience·Kai SunLixin Wei
Jul 1, 2014·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·R ZhouH Chen
Jul 16, 2014·Oncotarget·Zhiyu WangJianping Chen
Aug 1, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Meng-meng LvJian-hua Zhao
Aug 15, 2014·British Journal of Cancer·H GhebehM Al-Alwan
Aug 15, 2014·PloS One·Gi-Shih LienBing-Chang Chen
Aug 20, 2014·Autophagy·Michael J MorganAndrew Thorburn
Dec 24, 2014·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Yaghoub SafdariSafar Farajnia
Jun 5, 2015·Cancer Science·Qixing TanLun Song
Nov 14, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Liao NingLv Hai-Tong
Dec 17, 2015·Molecular Cancer·Xinfeng YuXianjun Qu
Apr 12, 2016·Cancer Biomarkers : Section a of Disease Markers·Xi GuWen-Hai Zhang
Jun 10, 2016·PloS One·Sofia Halin BergströmAnders Bergh
Jun 16, 2016·Biochemical and Biophysical Research Communications·Ji-Hoon ParkKyung-Soon Park
Dec 7, 2016·International Journal of Oncology·Ahmed Abdelbaset-IsmailMariusz Z Ratajczak
May 2, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jia-Teng ZhongZhi-Wei Feng
Jun 11, 2017·Scientific Reports·Peng ZhangXin Ma
Jun 22, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yuehua LiXiaoping Wu
Nov 5, 2017·Oncotarget·Floriana MorgilloMichele Orditura
Citations
May 8, 2019·The Kaohsiung Journal of Medical Sciences·Zi-Ming HuangJia-Li Tao
Sep 11, 2019·In Vitro Cellular & Developmental Biology. Animal·Yanyan WangJunbo Ge
Nov 11, 2019·Breast Cancer Research and Treatment·Natasha TraceyValerie G Brunton
Feb 23, 2020·Nutrients·Maha Sellami, Nicola Luigi Bragazzi
May 28, 2020·Journal of Clinical Medicine·Giuseppe SferrazzoIgnazio Barbagallo
Sep 8, 2020·Journal of Zhejiang University. Science. B·Nan WenZheng-Gui Du
Oct 5, 2019·Food & Function·Guangwen ShuXukun Deng
Nov 14, 2019·Cancers·Cally J Ho, Sharon M Gorski
Apr 21, 2020·Cancer Cell International·He XinZhaoyu Liu
Oct 21, 2020·Cells·Katharina BublitzBurkhard Hinz
Oct 31, 2020·Oxidative Medicine and Cellular Longevity·Ying ZhangLihong Yin
Feb 10, 2021·Cell Death & Disease·Aniketh BishnuPritha Ray
Nov 4, 2020·Free Radical Biology & Medicine·Malgorzata KucinskaMarek Murias
Feb 20, 2021·PeerJ·Mengya WangLiping Sun
Apr 4, 2021·Cancers·Hong-Tai TzengI-Tsu Chyuan
Jun 3, 2021·Antioxidants·Mariapaola NittiAnna Lisa Furfaro
Jul 9, 2021·OncoTargets and Therapy·Zimeng WuJie Lu