Heme oxygenase-carbon monoxide (HO-CO) system in rat uterus: effect of sexual steroids and prostaglandins

The Journal of Steroid Biochemistry and Molecular Biology
Maximiliano CellaAna M Franchi

Abstract

Pregnancy maintenance is a very complex phenomenon, and the mechanisms that allow the survival of the fetus within the maternal uterus are still poorly understood. Our objectives were to analyze heme oxygenase (HO) activity and expression in the pregnant rat and to study its association with steroid hormones and prostaglandins. Uterine tissues were obtained from non-pregnant and from time-mated rats at days 5, 13, 18-22 of pregnancy and postpartum. HO activity was significantly higher at days 5 and 20 while HO-1 protein levels measured by Western blot, were significantly elevated from days 19 to 22. In ovariectomized rats, estrogen and progesterone in estrogenized animals increased HO activity and expression. Cyclooxygenase inhibitors augmented HO activity and HO-1 expression. Pre-incubation with prostaglandin F2alpha (PGF2alpha) diminished the enzymatic activity in ovariectomized rat uterus. Tin protoporphyrin IX, an HO inhibitor, significantly decreased uterine cGMP accumulation. Bilirubin decreased uterine thiobarbituric acid substances levels (an index of lipid peroxidation). These results demonstrate a uterine gestational pattern of HO activity and expression in the rat. In addition, these results suggest that uterine HO a...Continue Reading

References

Jun 1, 1979·Analytical Biochemistry·H OhkawaK Yagi
Sep 24, 1990·European Journal of Biochemistry·B BrüneV Ullrich
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S M Keyse, R M Tyrrell
Jan 1, 1988·Drug and Chemical Toxicology·H Lin, J J McGrath
Oct 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·R TenhunenR Schmid
Sep 1, 1981·Biochemistry·R B FrydmanB Frydman
Jan 15, 1993·Science·A VermaS H Snyder
Nov 1, 1993·American Journal of Obstetrics and Gynecology·S M SladekJ M Roberts
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·T MoritaS Kourembanas
Apr 1, 1993·Journal of Neurochemistry·J F Ewing, M D Maines
Jan 12, 1996·Biochemical Pharmacology·G EngelhardtR Utzmann
Jan 12, 1996·Biochemical Pharmacology·G EngelhardtR Utzmann
Dec 1, 1996·Pediatric Research·P A DenneryV Shokoohi
Jan 1, 1997·Annual Review of Pharmacology and Toxicology·M D Maines
Jul 15, 1997·European Journal of Biochemistry·W K McCoubreyM D Maines
May 1, 1997·Brain Research. Brain Research Protocols·J F Ewing, M D Maines
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·S DoréS H Snyder
Sep 17, 1999·The American Journal of Pathology·A BarberF Lyall
Feb 24, 2001·Journal of Physiology and Biochemistry·M CriadoA Esteller
Aug 15, 2001·The Journal of Clinical Endocrinology and Metabolism·W TschugguelJ C Huber
Sep 14, 2001·Antioxidants & Redox Signaling·T J HuangM D Maines
Oct 16, 2001·The Journal of Clinical Endocrinology and Metabolism·N YoshikiT Aso
Dec 13, 2002·Fertility and Sterility·Françoise Casanas-RouxJacques Donnez
May 9, 2003·Pediatric Research·Doron KreiserPhyllis A Dennery
Aug 21, 2003·Biochemical and Biophysical Research Communications·Nina GrosserHenning Schröder
Oct 8, 2003·Current Pharmaceutical Design·M J AlcarazM I Guillén
Sep 1, 2004·The Journal of Steroid Biochemistry and Molecular Biology·M FarinaA Franchi

❮ Previous
Next ❯

Citations

Mar 29, 2011·Alcoholism, Clinical and Experimental Research·Catherine A VandeVoortAlan J Conley
Mar 25, 2014·Frontiers in Endocrinology·Maria Laura ZenclussenAna Claudia Zenclussen
Sep 26, 2006·Journal of Neuroscience Research·Cesare MancusoVittorio Calabrese
Sep 29, 2020·Frontiers in Physiology·Enkhjargal BudbazarFrancesca Seta

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.