Heme oxygenase expression and activity in immortalized hypothalamic neurons GT1-7

Neuroscience Letters
Nadia MoresCesare Mancuso

Abstract

Heme oxygenase (HO), the main enzyme deputed to heme metabolism, has been identified as two main isoforms called HO-1 and HO-2. HO-1 is inducible and plays a main role in the cellular oxidant/antioxidant balance whereas HO-2 is constitutive and involved in the physiological metabolism of heme. However, it is noteworthy to mention that HO contribute to the regulation of the hypothalamic release of neuropeptides such as corticotrophin-releasing hormone and arginine-vasopressin and could modulate the pulsatile release of gonadotropin releasing hormone (GnRH). GT1-7 cells are immortalized hypothalamic neurons and a valuable tool to evaluate hypothalamic neuroendocrine control of reproduction. The aim of this work was to investigate and characterize the presence of HO isoforms in the GT1-7 hypothalamic neurons. Hemin, a well-known inducer of HO-1, significantly increased HO activity, whereas dexamethasone did not modify HO-2 activity. Moreover, hemin and DEX, in combination, did not have any additive effect on HO activity in GT1-7 neurons. Furthermore, basal HO-1 immunoreactivity identified in GT1-7 cells, was significantly up-regulated by hemin. Conversely, no HO-2 immunoreactivity was detected. Taken together, these results sugges...Continue Reading

References

Jul 15, 1997·European Journal of Biochemistry·W K McCoubreyM D Maines
Dec 24, 1997·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·A GrossmanM A Satta
Sep 4, 2004·Antioxidants & Redox Signaling·Cesare Mancuso
Dec 17, 2005·Antioxidants & Redox Signaling·Mahin D Maines
May 9, 2006·Antioxidants & Redox Signaling·Cesare MancusoGiovambattista Pani
Oct 13, 2006·Antioxidants & Redox Signaling·Vittorio CalabreseD A Butterfield
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Cesare MancusoVittorio Calabrese
Nov 30, 2006·Redox Report : Communications in Free Radical Research·Cesare MancusoVittorio Calabrese
Dec 19, 2006·Stress : the International Journal on the Biology of Stress·James S Kinsey-JonesKevin T O'Byrne
Mar 1, 1996·Trends in Endocrinology and Metabolism : TEM·L Z KrsmanovicK J Catt

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