Heme structures of five variants of hemoglobin M probed by resonance Raman spectroscopy

Biochemistry
Y JinT Kitagawa

Abstract

The alpha-abnormal hemoglobin (Hb) M variants show physiological properties different from the beta-abnormal Hb M variants, that is, extremely low oxygen affinity of the normal subunit and extraordinary resistance to both enzymatic and chemical reduction of the abnormal met-subunit. To get insight into the contribution of heme structures to these differences among Hb M's, we examined the 406.7-nm excited resonance Raman (RR) spectra of five Hb M's in the frequency region from 1700 to 200 cm(-1). In the high-frequency region, profound differences between met-alpha and met-beta abnormal subunits were observed for the in-plane skeletal modes (the nu(C=C), nu(37), nu(2), nu(11), and nu(38) bands), probably reflecting different distortions of heme structure caused by the out-of-plane displacement of the heme iron due to tyrosine coordination. Below 900 cm(-1), Hb M Iwate [alpha(F8)His --> Tyr] exhibited a distinct spectral pattern for nu(15), gamma(11), delta(C(beta)C(a)C(b))(2,4), and delta(C(beta)C(c)C(d))(6,7) compared to that of Hb M Boston [alpha(E7)His --> Tyr], although both heme irons are coordinated by Tyr. The beta-abnormal Hb M variants, namely, Hb M Hyde Park [beta(F8)His --> Tyr], Hb M Saskatoon [beta(E7)His --> Tyr], a...Continue Reading

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Citations

Jun 16, 2012·The Journal of Biological Chemistry·Célia Caillet-SaguyAnne Lecroisey
Aug 25, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Yayoi AkiTeizo Kitagawa
May 27, 2014·Journal of Inorganic Biochemistry·Shin-Ichi OzakiKoichiro Ishimori
Jul 18, 2014·Revista brasileira de hematologia e hemoterapia·Marcos Borato Viana, André Rolim Belisário
Apr 12, 2017·Applied Spectroscopy·Chad G AtkinsRobin F B Turner
Dec 29, 2007·The Journal of Biological Chemistry·Célia Caillet-SaguyAnne Lecroisey
Jan 26, 2010·Analytical Chemistry·Manikantan Syamala KiranMitsuru Ishikawa
May 18, 2021·Computational and Structural Biotechnology Journal·Dietmar HammerschmidSylvia Dewilde
Jul 16, 2021·Biophysical Journal·Vaibhav A DixitShivam Kumar Vyas
Dec 10, 2016·The Journal of Physical Chemistry. B·Katarzyna M MarzecMalgorzata Baranska

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