Heme Uptake in Lactobacillus sakei Evidenced by a New Energy Coupling Factor (ECF)-Like Transport System.

Applied and Environmental Microbiology
E. VerplaetseMarie-Christine Champomier-Vergès

Abstract

Lactobacillus sakei is a nonpathogenic lactic acid bacterium and a natural inhabitant of meat ecosystems. Although red meat is a heme-rich environment, L. sakei does not need iron or heme for growth, although it possesses a heme-dependent catalase. Iron incorporation into L. sakei from myoglobin and hemoglobin was previously shown by microscopy and the L. sakei genome reveals the complete equipment for iron and heme transport. Here, we report the characterization of a five-gene cluster (from lsa1836 to lsa1840 [lsa1836-1840]) encoding a putative metal iron ABC transporter. Interestingly, this cluster, together with a heme-dependent catalase gene, is also conserved in other species from the meat ecosystem. Our bioinformatic analyses revealed that the locus might correspond to a complete machinery of an energy coupling factor (ECF) transport system. We quantified in vitro the intracellular heme in the wild type (WT) and in our Δlsa1836-1840 deletion mutant using an intracellular heme sensor and inductively coupled plasma mass spectrometry for quantifying incorporated 57Fe heme. We showed that in the WT L. sakei, heme accumulation occurs rapidly and massively in the presence of hemin, while the deletion mutant was impaired in heme...Continue Reading

References

Jan 1, 1981·Annual Review of Biochemistry·J B Neilands
Apr 18, 1998·Applied and Environmental Microbiology·C HertelW P Hammes
Jun 8, 2001·The Journal of Biological Chemistry·L LiJ Kaplan
Feb 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sarkis K MazmanianOlaf Schneewind
Feb 8, 2003·Science·Sarkis K MazmanianOlaf Schneewind
Sep 6, 2003·Applied and Environmental Microbiology·Carl-Alfred AlpertMonique Zagorec
Sep 15, 2004·International Journal of Food Microbiology·L VermeirenJ Debevere
Sep 30, 2004·Journal of Food Protection·F DevlieghereJ Debevere
Jun 28, 2005·Nucleic Acids Research·Johannes SödingAndrei N Lupas
Nov 8, 2005·Nature Biotechnology·Stéphane ChaillouMonique Zagorec
Jun 1, 1996·Applied and Environmental Microbiology·R LauretM Zagorec
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jochen WuergesLucio Randaccio
Oct 3, 2006·Journal of Bacteriology·Anthony W MaressoOlaf Schneewind
Mar 28, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Michelle L ReniereEric P Skaar
Aug 7, 2007·Applied and Environmental Microbiology·Mark S TurnerPhilip M Giffard
Oct 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·F S MathewsN Sukumar
Oct 22, 2008·Journal of Bacteriology·Dmitry A RodionovThomas Eitinger
Jun 2, 2009·Microbial Cell Factories·Rob BrooijmansJeroen Hugenholtz
Aug 4, 2010·Infection and Immunity·Laura L Anzaldi, Eric P Skaar
Jan 8, 2011·Current Opinion in Biotechnology·Delphine LechardeurAlexandra Gruss
May 4, 2011·Biochemistry·Joseph N RobertsLindsay D Eltis
Oct 11, 2012·Advances in Microbial Physiology·Alexandra GrussDelphine Lechardeur
Apr 16, 2013·Nature·Tingliang WangYigong Shi
Jul 13, 2013·The Journal of Biological Chemistry·Evelyne FurgerAndrea E Prota
Jul 22, 2014·Molecular Microbiology·Laetitia JoubertDelphine Lechardeur
Aug 26, 2014·PloS One·Erin S HonsaSarah K Highlander
Dec 17, 2014·Proceedings of the National Academy of Sciences of the United States of America·Minhua ZhangPeng Zhang
Jun 21, 2015·Biochimica Et Biophysica Acta·Elise Abi-KhalilChristina Nielsen-Leroux
Mar 29, 2016·Journal of Molecular Biology·Jacob E Choby, Eric P Skaar
Jun 21, 2016·Current Protocols in Bioinformatics·Benjamin Webb, Andrej Sali

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Citations

Feb 6, 2021·Gut Microbes·Yohannes SeyoumChristèle Humblot
Aug 20, 2021·Proceedings of the National Academy of Sciences of the United States of America·Chancievan ThangaratnarajahDirk J Slotboom

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