Hemicelluloses of ragi (finger millet, Eleusine coracana, Indaf-15): isolation and purification of an alkali-extractable arabinoxylan from native and malted hemicellulose B

Journal of Agricultural and Food Chemistry
M V S S T Subba Rao, G Muralikrishna

Abstract

Hemicelluloses (A and B) were isolated from an Indo-African hybrid variety of finger millet (ragi, Eleusine coracana) by extracting the starch-free residue with 10% sodium hydroxide under a continuous stream of nitrogen, and changes in their sugar composition during malting for 96 h were studied. Hemicellulose B, obtained in higher yield from both native (N) and malted (M) flours, was found to be completely soluble in water, richer in uronic acid, and more viscogenic than hemicelullose A. Fractional precipitation of hemicellulose B by ammonium sulfate resulted in four precipitable fractions (F-60, F-70, F-80, and F-100) and a nonprecipitable (NP) fraction varying in their yield and arabinose, xylose, galactose, and glucose contents. A progressive increase in the pentose-to-hexose ratio (P:H) from 0.42:1.0 in F-60 to 1.94:1.0 in NP was observed in native hemicellulose B fractions; however, in malted hemicellulose B the P:H ratio increased from 0.43:1.0 in F-60 to 1.56:1.0 in F-80 and then decreased to 1.13:1.0 in NP. The major fraction, F-70 (N, 44.5%; M, 38.5%), was separated into eight subfractions on DEAE-cellulose by successive elution with water, ammonium carbonate (AC) (0.1, 0.2, and 0.3 M AC), and sodium hydroxide (0.1 an...Continue Reading

References

May 2, 2002·Journal of Agricultural and Food Chemistry·Stephen Mbithi-MwikyaAndre Huyghebaert
Oct 17, 2002·Journal of Agricultural and Food Chemistry·Chilkunda D Nandini, Paramahans V Salimath

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Citations

Apr 26, 2012·Journal of Agricultural and Food Chemistry·Wei LiuKecheng Li
Jul 27, 2007·Critical Reviews in Food Science and Nutrition·G Muralikrishna, M V S S T Subba Rao
Feb 7, 2012·Biotechnology Advances·Feng PengRun-Cang Sun
Nov 28, 2013·Carbohydrate Polymers·M R Savitha Prashanth, G Muralikrishna
Jan 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaopeng PengQuanzi Li
Jan 28, 2021·Journal of the Science of Food and Agriculture·Uma Maheshwari Srinivasa, Madeneni Madhava Naidu
Mar 23, 2010·Journal of Agricultural and Food Chemistry·Feng PengRun-Cang Sun

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