Hemicentin 1 influences podocyte dynamic changes in glomerular diseases

American Journal of Physiology. Renal Physiology
Barbara ToffoliBéatrice Desvergne

Abstract

Different complex mechanisms control the morphology of podocyte foot processes and their interactions with the underlying basement membrane. Injuries to this system often cause glomerular dysfunction and albuminuria. The present study aimed at identifying early markers of glomerular damage in diabetic nephropathy. For this purpose, we performed a microarray analysis on kidneys of 3-wk-old peroxisome proliferator-activated receptor-γ (PPARγ)-null and AZIP/F1 mice, which are two models of diabetic nephropathy due to lipodystrophy. This was followed by functional annotation of the enriched clusters of genes. One of the significant changes in the early stages of glomerular damage was the increase of hemicentin 1 (HMCN1). Its expression and distribution were then studied by real-time PCR and immunofluorescence in various models of glomerular damage and on podocyte cell cultures. HMCN1 progressively increased in the glomeruli of diabetic mice, according to disease severity, as well as in puromycin aminonucleoside (PA)-treated rats. Studies on murine and human podocytes showed an increased HMCN1 deposition upon different pathological stimuli, such as hyperglycemia, transforming growth factor-β (TGF-β), and PA. In vitro silencing studi...Continue Reading

References

Oct 24, 1998·Genes & Development·J MoitraC Vinson
May 31, 2001·Kidney International·M W SteffesUNKNOWN International Diabetic Nephropathy Study Group
Apr 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Takeshi ImaiDaniel Metzger
Oct 22, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Takayoshi SuganamiKazuwa Nakao
Jun 3, 2005·Current Opinion in Nephrology and Hypertension·Simone Monika Blattner, Matthias Kretzler
Jun 7, 2005·Journal of the American Society of Nephrology : JASN·Francesco P Schena, Loreto Gesualdo
Aug 19, 2005·Journal of the American Society of Nephrology : JASN·Terry SamuelJohn F Bertram
Oct 26, 2005·Human Molecular Genetics·Ian Smyth, Peter Scambler
Apr 6, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maria Pia RastaldiGiuseppe D'Amico
Oct 4, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Xuehong XuBruce E Vogel
Oct 13, 2006·Cell Research·Bruce E VogelXuehong Xu
Jun 27, 2007·Human Molecular Genetics·Cheryl L ThompsonSudha K Iyengar
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Jul 21, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Jing MiaoJie Ding
Mar 20, 2010·Clinical Journal of the American Society of Nephrology : CJASN·Sulgi KimSharon G Adler
Sep 3, 2010·Endocrinology·Karim NadraBéatrice Desvergne
Jan 11, 2011·Current Biology : CB·Xuehong Xu, Bruce E Vogel
Feb 11, 2011·Diabetes/metabolism Research and Reviews·Nicole StiegerMario Schiffer
Mar 14, 2012·Experimental Cell Research·Jeffrey H Miner
Jul 10, 2012·Disease Models & Mechanisms·Cristina Martínez-GarcíaGema Medina-Gomez
Dec 14, 2012·American Journal of Physiology. Renal Physiology·Wilhelm KrizKevin V Lemley
Jan 11, 2013·Physiological Reviews·Josephine M Forbes, Mark E Cooper
Mar 8, 2013·American Journal of Physiology. Renal Physiology·Nirupama ChandelPravin C Singhal
Apr 24, 2013·Nature Reviews. Nephrology·Paul Thomas BrinkkoetterThomas Benzing
Jun 19, 2013·Nature Reviews. Nephrology·Jung Hee Suh, Jeffrey H Miner
Jun 28, 2013·Cell & Bioscience·Xuehong XuWilliams B Isaacs
Jan 18, 2014·Journal of the American Society of Nephrology : JASN·Rachel LennonMartin J Humphries
Jan 18, 2014·Journal of the American Society of Nephrology : JASN·Adam ByronRachel Lennon
May 2, 2014·Advances in Chronic Kidney Disease·Jay C JhaMark E Cooper
Jun 22, 2014·Cardiovascular Research·Arpita ChowdhuryGregor Theilmeier
Jul 15, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Alexandra NabaRichard O Hynes
Oct 29, 2015·The New England Journal of Medicine·Mauro TancrediMarcus Lind
Mar 31, 2016·Current Opinion in Nephrology and Hypertension·Rachel Lennon, Salman Hosawi

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Citations

Feb 9, 2020·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Meei-Hua LinJeffrey H Miner

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Methods Mentioned

BETA
transgenic
ELISA
biopsies
PCR
transfection

Software Mentioned

ImageJ
GraphPad
DAVID
R
Prism
DAVID Functional Annotation Clustering
Bioconductor

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