Hemilipin, a novel Hemiscorpius lepturus venom heterodimeric phospholipase A2, which inhibits angiogenesis in vitro and in vivo

Toxicon : Official Journal of the International Society on Toxinology
Imen JridiLamia Borchani

Abstract

Phospholipases A2 (PLA2) are enzymes which specifically hydrolyze the sn-2 acyl ester bond of phospholipids producing free fatty acids and lysophospholipids. The secreted PLA2 (sPLA2) are the most common types of PLA2 purified from the snake venom, mammalian pancreatic juice and other sources. They display a variety of toxic actions and biological activities, including antitumoral and antiangiogenic effects. In this study, we report the isolation, characterization and the antiangiogenic activity of Hemilipin, a novel sPLA2 extracted from Hemiscorpius lepturus venom, the most dangerous scorpion in Iran. Hemilipin was purified by HPLC and analyzed by MALDI TOF/MS. The primary structure was determined by EDMAN degradation method and the PLA2 activity by titration of fatty acids released from the egg yolk phospholipids. Its antiangiogenic activity was studied in vitro by evaluating effects on apoptosis, Matrigel angiogenesis, migration and adhesion of human umbilical vein endothelial cells (HUVECs) and human pulmonary artery endothelial cells (HPAECs) and in vivo by the chorioallantoic membrane (CAM) assay. Mass spectrometry profile showed that Hemilipin is heterodimeric and the PLA2 test demonstrated its strong hydrolytic activity...Continue Reading

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Citations

May 12, 2017·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Babak MostafazadehHamid Reza Khoddami Vishteh
Feb 16, 2019·Molecular & Cellular Proteomics : MCP·Carolina MöllerFrank Marí
Mar 7, 2018·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Rouhullah DehghaniMalihe Mohammadi
Dec 14, 2019·Expert Review of Proteomics·Jimena I Cid-UribeLourival D Possani
Dec 17, 2017·Oncotarget·Rui MaHang Fai Kwok
Jul 26, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Seyed Mahdi Kazemi, Jean-Marc Sabatier
Dec 20, 2020·Toxicon : Official Journal of the International Society on Toxinology·Masahiro MiyashitaHisashi Miyagawa
May 1, 2019·Molecular & Cellular Proteomics : MCP·Carolina MöllerFrank Marí
Jun 1, 2020·Toxicon : Official Journal of the International Society on Toxinology·Najeh Krayem, Youssef Gargouri
Feb 28, 2018·Toxicon : Official Journal of the International Society on Toxinology·Najeh KrayemYoussef Gargouri
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sedigheh Bagheri-ZiariKamran Pooshang Bagheri
Nov 6, 2018·International Journal of Biological Macromolecules·Edigar H V DiasFábio de Oliveira

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