Hemin induces mitophagy in a leukemic erythroblast cell line

Biology of the Cell
Claudio Marcelo FaderMaría Isabel Colombo

Abstract

In eukaryotic cells, autophagy is considered a lysosomal catabolic process which participates in the degradation of intracellular components in a vacuolar structure termed autolysosome. This pathway plays a significant role in the erythropoiesis process, contributing to the clearance of some organelles (such as mitochondria) that are not necessary in the mature red blood cells. Nevertheless, the role of autophagy in erythrocyte maturation has not been fully established. Here, we have demonstrated that hemin (a physiological erythroid maturation stimulator) is able to induce the expression of critical autophagic genes (i.e., Map1a1b (LC3), Beclin-1 gen, Atg5) in an erythroleukemia cell type. We have also shown that hemin increased the size of autophagic vacuoles which were labelled with LC3 and the degradative lysosomal marker dye quenched-bovine serum albumin. In addition, we have determined by Western blot a rise in the lipidated form of the autophagic protein LC3 (i.e., LC3-II) upon hemin treatment. Moreover, we provide evidence that hemin induces mitochondrial membrane depolarisation and that mitochondria sequestration by autophagy requires the active form of the NIX protein. We have found that the physiological erythroid ma...Continue Reading

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Citations

Feb 2, 2019·Cells·Mojgan Djavaheri-MergnyMagali Humbert
Jun 10, 2017·The EMBO Journal·Lorenzo GalluzziGuido Kroemer
Jan 10, 2018·Frontiers in Physiology·Martina MorasMariano A Ostuni
Dec 14, 2018·Bioscience Reports·Ruben Adrian GrossoClaudio Marcelo Fader
Jan 1, 2021·Frontiers in Immunology·Vanessa Araujo Gomes SantaterraErich Vinicius De Paula
Jul 2, 2019·Journal of Molecular Biology·Sebastian E Koschade, Christian H Brandts

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