Heminested reverse-transcriptase polymerase chain reaction (hnRT-PCR) as a tool for rabies virus detection in stored and decomposed samples.

BMC Research Notes
Danielle B AraújoJane Megid

Abstract

The use of methods, both sensitive and specific, for rabies diagnosis are important tools for the control and prophylaxis of the disease. Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) has been used in rabies diagnosis with good results, even in decomposed materials. Additionally, molecular techniques have been used for epidemiological studies and to gain a better knowledge of viral epidemiology. The aim of this work was to evaluate the RT-PCR and hnRT-PCR for rabies virus detection in original tissues stored at -20 degrees C for different periods considering their use for rabies virus detection in stored and decomposed samples. RT-PCR and hnRT-PCR were evaluated in 151 brain samples from different animal species, thawed and left at room temperature for 72 hours for decomposition.The RT-PCR and hnRT-PCR results were compared with previous results from Direct Fluorescent Antibody Test and Mouse Inoculation Test. From the 50 positive fresh samples, 26 (52%) were positive for RT-PCR and 45 (90%) for hnRT-PCR. From the 48 positive decomposed samples, 17 (34, 3%) were positive for RT-PCR and 36 (75%) for hnRT-PCR. No false-positives results were found in the negatives samples evaluated to the molecular techniques. These re...Continue Reading

References

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Citations

Dec 3, 2013·Indian Journal of Virology : an Official Organ of Indian Virological Society·R P Aravindh BabuN D J Chandran
Sep 30, 2009·PLoS Neglected Tropical Diseases·Anthony R FooksThomas Müller
Mar 11, 2010·Expert Review of Molecular Diagnostics·Supaporn Wacharapluesadee, Thiravat Hemachudha
May 26, 2012·Journal of Virological Methods·Supaporn WacharapluesadeeThiravat Hemachudha
Oct 19, 2013·Journal of Medical Virology·Reeta Subramaniam ManiSusarla Krishna Shankar
Dec 19, 2013·TheScientificWorldJournal·Reeta Subramaniam Mani, Shampur Narayan Madhusudana
Jul 11, 2014·Journal of Virological Methods·Lorraine M McElhinneyAnthony R Fooks
Mar 24, 2016·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Veasna DuongPhilippe Buchy
Apr 9, 2011·Journal of Virological Methods·Angélica Cristine de Almeida CamposSilvana Regina Favoretto
May 10, 2018·Archives of Virology·Juliana Galera CastilhoCarla Isabel Macedo

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Methods Mentioned

BETA
Polymerase Chain Reaction
PCR
1
×
electrophoresis

Software Mentioned

BRITO

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