Abstract
The activation and adhesion of platelets or whole blood exposed to chitosan (CH) grafted surfaces is used to evaluate the hemocompatibility of biomaterials. The biomaterial surfaces are polyurethane (PU) tubes grafted with an inner poly(acrylic acid) (PAA) and an outer CH or quaternary ammonium modified CH (CH-Q) brush. The CH, CH-Q and PAA grafted layers were characterized by ellipsometry and fluorescence microscopy. Material wear tests demonstrate that CH (CH-Q) is stably grafted onto PU tubes upon exposure to saline solution for 7 days. Using quartz-crystal microbalances with dissipation (QCM-D), in-situ adsorption of blood plasma proteins on CH and CH-Q compared to a silicon oxide control was measured. The QCM-D results showed that the physically adsorbed plasma protein layer on CH-Q and CH surfaces is softer and more viscous than the protein layer on the SiO2 surface. The CH-Q layer thus has the weakest interaction with plasma proteins. Whole blood and platelet adhesion was reduced by ~92% on CH-Q, which showed the weakest interaction with plasma protein but more viscous adsorbed plasma protein layer, compared to SiO2. Last, to examine the biologic response of platelets and neutrophils to biomaterial surfaces, CH (CH-Q)/PA...Continue Reading
References
Jan 1, 1987·Annals of the New York Academy of Sciences·R W ColmanL H Edmunds
Oct 1, 1986·Journal of Biomedical Materials Research·Y ItoY Imanishi
Jul 1, 1973·The Journal of Infectious Diseases·A I BraudeC E Davis
May 3, 1974·Science·L Vroman
Aug 1, 1993·Biomaterials·I K KangH B Lee
Apr 1, 1996·Biomaterials·I K KangY K Sung
Aug 28, 1998·Biomaterials·G M BernaccaD J Wheatley
Feb 26, 2000·Annals of Internal Medicine·L A Mermel
Jan 5, 2002·Biomaterials·Norbert WeberGerhard Ziemer
Jan 17, 2002·Analytical Chemistry·F HöökH Elwing
Apr 23, 2004·Current Opinion in Structural Biology·Jeffrey J Gray
Oct 17, 2007·Journal of Biomedical Materials Research. Part a·Emmanuelle Monchaux, Patrick Vermette
Feb 22, 2008·Current Topics in Medicinal Chemistry·Saravanababu MurugesanRobert J Linhardt
Jan 27, 2009·Biomaterials·Erwin A Vogler, Christopher A Siedlecki
Feb 1, 2011·Colloids and Surfaces. B, Biointerfaces·Irene Y TsaiDavid M Eckmann
Mar 25, 2011·Biomaterials·Stanley J StachelekRobert J Levy
Apr 12, 2011·Soft Matter·Irene Y TsaiDavid M Eckmann
May 24, 2011·Journal of Materials Science. Materials in Medicine·Stefan SinnHans P Wendel
Sep 8, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Hyun-Su LeeRussell J Composto
May 23, 2012·Biomaterials·Matthew J FinleyStanley J Stachelek
Aug 1, 2012·Journal of Biomedical Materials Research. Part a·Hyun-Su LeeDavid M Eckmann
Sep 19, 2012·ACS Applied Materials & Interfaces·Xiao LiuWeiwei Zhu
Dec 5, 2012·Journal of Materials Chemistry·Hyun-Su LeeRussell J Composto
Mar 26, 2013·Colloids and Surfaces. B, Biointerfaces·David M EckmannRussell J Composto
Citations
Feb 22, 2017·ACS Applied Materials & Interfaces·Prathima C NalamRussell J Composto
Mar 7, 2020·Macromolecular Rapid Communications·Wei SunHong Chen
Mar 14, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Dongsheng TanQiang Fu
Sep 24, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Tong LiYong-Kuan Gong
Jan 15, 2015·Biomacromolecules·Hyun-Su LeeRussell J Composto