Hemodynamic and histological effects of traumatic brain injury in eNOS-deficient mice

Journal of Neurotrauma
C LundbladPeter Bentzer

Abstract

Microvascular dysfunction in the brain, characterized by vasoconstriction, vascular occlusion, and disruption of the blood brain barrier, may adversely affect outcome following traumatic brain injury (TBI). Because of its vasodilating and antiaggregative properties, nitric oxide (NO) produced by nitric oxide synthase in the endothelium (eNOS) is a key regulator of vascular homeostasis. The objective of the present study was to evaluate the role of eNOS in vascular disturbances and histological outcome in the brain following TBI. Cortical blood flow ([(14)C]-iodoantipyrine technique), number of perfused capillaries (FITC-dextran technique), brain water content (wet vs. dry weight), and the transfer constant (K(i)) for [(51)Cr]-EDTA, reflecting permeability, were analyzed 3 h and 24 h after a controlled cortical impact injury (CCI) in eNOS-deficient (eNOS-KO) and wild-type (WT) mice. Cortical contusion volume and cell count in the hippocampus were evaluated 3 weeks after injury. Blood flow in the injured cortex decreased in both groups following trauma. There were no significant differences between the groups at 3 h, but blood flow was lower in eNOS-KO mice than in WT mice 24 h after trauma. Brain water content was higher in the ...Continue Reading

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Citations

Dec 4, 2010·Circulation Research·Susan A AustinZvonimir S Katusic
Nov 5, 2014·Journal of Neurotrauma·Susanne M SchwarzmaierNikolaus Plesnila
Oct 28, 2014·Frontiers in Immunology·Jovany Cruz NavarroClaudia S Robertson
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Feb 20, 2018·Translational Neurodegeneration·Sandesh PanthiKripa Gautam
Jul 12, 2019·Journal of Neuroinflammation·Richard L JayarajGary A Rosenberg
Dec 2, 2020·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Irina J LenzNicole A Terpolilli
Jan 20, 2021·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Marius M MaderJuliane Hannemann
Feb 23, 2021·ACS Pharmacology & Translational Science·Rachel C KnoppGregory R J Thatcher

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Methods Mentioned

BETA
PCR
X-ray

Software Mentioned

STAT
Sigma Stat
SigmaScan Pro
CAST

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