Hemodynamic effects of acute and repeated exposure to raloxifene in ovariectomized sheep

American Journal of Physiology. Heart and Circulatory Physiology
Willie D ZomaKenneth E Clark

Abstract

We hypothesize that administration of acute and daily doses of raloxifene will have significant effects on ovine coronary and uterine hemodynamics and that these changes are estrogen receptor dependent. Eleven ovariectomized sheep were instrumented to measure mean arterial pressure, heart rate (HR), cardiac output (CO), and coronary (CBF) and uterine artery blood flows (UBF). A dose-response curve was generated for raloxifene (1, 3, and 10 microg/kg) and compared with a standard dose of estradiol-17beta (1 microg/kg) given intravenously. In a second group of animals, raloxifene (10 microg.kg-1.day-1) was administered intravenously for 14 consecutive days, and cardiovascular responses were compared with a group of animals administered estradiol-17beta (10 microg/kg) daily for the same period. To determine whether raloxifene-related vascular responses were estrogen receptor (ER) mediated, the animals were pretreated with estrogen antagonist ICI-182,780 given intravenously. Finally, RT-PCR was preformed to determine the presence of ERalpha and ERbeta mRNA in ovine coronary and uterine vessels. Raloxifene increased CBF and UBF dose dependently with a parallel decrease in the associated vascular resistances. Acute cardiovascular res...Continue Reading

References

Sep 12, 1991·The New England Journal of Medicine·M J StampferC H Hennekens
Nov 1, 1980·American Journal of Obstetrics and Gynecology·W H ClewellG Meschia
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·C P WeinerS Moncada
May 26, 1995·Biochemical Pharmacology·A E Wakeling
May 30, 1996·The American Journal of Cardiology·T MünzelD G Harrison
Nov 1, 1996·The Journal of Clinical Investigation·C R RosenfeldR R Magness
May 1, 1997·Human Reproduction·C BullettiF Franceschetti
Jun 1, 1997·The American Journal of Physiology·D J DarkowR E White
Aug 1, 1997·European Journal of Obstetrics, Gynecology, and Reproductive Biology·U LangK E Clark
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·R H KarasM E Mendelsohn
Jan 29, 2000·American Journal of Obstetrics and Gynecology·P KamaliJ F Clapp
Mar 30, 2000·American Journal of Obstetrics and Gynecology·W D ZomaK E Clark
Aug 18, 2000·The Journal of Clinical Endocrinology and Metabolism·T Simoncini, A R Genazzani
Feb 13, 2001·The Journal of Steroid Biochemistry and Molecular Biology·M E Mendelsohn
May 12, 2001·American Journal of Obstetrics and Gynecology·H R DeitchK E Clark
Sep 8, 2001·The American Journal of Cardiology·L MoscaP W Anderson
Feb 20, 2002·JAMA : the Journal of the American Medical Association·Elizabeth Barrett-ConnorUNKNOWN MORE Investigators (Multiple Outcomes of Raloxifene Evaluation)
Jul 2, 2002·JAMA : the Journal of the American Medical Association·Deborah GradyUNKNOWN HERS Research Group
Jul 19, 2002·JAMA : the Journal of the American Medical Association·Jacques E RossouwUNKNOWN Writing Group for the Women's Health Initiative Investigators
Aug 16, 2002·American Journal of Physiology. Heart and Circulatory Physiology·John L MershonKenneth E Clark
Feb 20, 2003·American Journal of Obstetrics and Gynecology·Philip M SarrelDavid L Katz
Mar 22, 2003·Clinical Pharmacokinetics·Karla C MorelloMichael W DeGregorio
Aug 9, 2003·The New England Journal of Medicine·JoAnn E MansonUNKNOWN Women's Health Initiative Investigators
Apr 15, 2004·JAMA : the Journal of the American Medical Association·Garnet L AndersonUNKNOWN Women's Health Initiative Steering Committee

❮ Previous
Next ❯

Citations

Jul 12, 2011·European Journal of Pharmacology·Noboru Toda, Hiroshi Toda
Mar 6, 2019·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·T N AminD Jurkovic

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.