Hemodynamic forces enhance decidualization via endothelial-derived prostaglandin E2 and prostacyclin in a microfluidic model of the human endometrium.

Human Reproduction
Juan S GneccoKevin G Osteen

Abstract

Does the uterine vasculature play a localized role in promoting stromal cell decidualization in the human endometrium? Our study demonstrated that hemodynamic forces induced secretion of specific endothelial cell-derived prostanoids that enhanced endometrial perivascular decidualization via a paracrine mechanism. Differentiation of stromal cell fibroblasts into the specialized decidua of the placenta is a progesterone-dependent process; however, histologically, it has long been noted that the first morphological signs of decidualization appear in the perivascular stroma. These observations suggest that the human endometrial vasculature plays an active role in promoting stromal differentiation. Primary human endometrial stromal cells were co-cultured for 14 days with primary uterine microvascular endothelial cells within a microfluidic Organ-on-Chip model of the endometrium. Cultures were maintained with estradiol and a progestin, with or without continuous laminar perfusion to mimic hemodynamic forces derived from the blood flow. Some cultures additionally received exogenous agonist-mediated challenges. Decidualization in the microfluidic model was assessed morphologically and biochemically. ELISA was used to examine the cultur...Continue Reading

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Citations

Apr 30, 2020·Tissue Engineering. Part a·Hannes CampoJ Julie Kim
Dec 20, 2019·Acta Biochimica Et Biophysica Sinica·Yifei SangMeirong Du
Aug 11, 2020·Bio-design and Manufacturing·Maria E Zubizarreta, Shuo Xiao
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Aug 8, 2021·Human Reproduction·Jungho AhnYoun-Jung Kang
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Oct 31, 2021·American Journal of Reproductive Immunology : AJRI·Guillermina Girardi, Andrew A Bremer

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Methods Mentioned

BETA
hysterectomy
biopsy
enzyme-linked immunosorbent assays
ELISAs

Software Mentioned

GraphPad Prism

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