Hemoglobin adducts of benzene oxide in neonatal and adult dried blood spots.

Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology
William E FunkStephen M Rappaport

Abstract

Adducts of reactive chemicals with hemoglobin (Hb) or human serum albumin can be used as biomarkers of internal doses of carcinogens. Because dried blood spots are easier to collect and store than conventional venous blood samples, they encourage applications of biomarkers of exposure in large epidemiologic studies. In addition, neonatal dried blood spot can be used to investigate chemical exposures in utero. Here, we report a simple method to isolate Hb from dried blood spot with high recovery and purity using the addition of ethanol to aqueous dried blood spot extracts. To prove the concept that dried blood spot-derived proteins can be used to assay for adducts, we measured Hb adducts of benzene oxide, a reactive metabolite of the ubiquitous air pollutant benzene in nine neonatal and nine adult dried blood spots (from volunteer subjects), using a gas chromatography-mass spectrometry method that we had previously developed. For comparison, benzene oxide-Hb adducts were measured in the same nine adult subjects using Hb that had been isolated and purified using our conventional method for venous blood. The geometric mean of benzene oxide-Hb levels in all dried blood spot samples ranged from 27.7 to 33.1 pmol/g globin. Neither of...Continue Reading

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Citations

May 11, 2012·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Joachim D Pleil
Aug 13, 2009·Environmental Health Perspectives·Martyn T Smith, Stephen M Rappaport
May 22, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·J D PleilM C Madden
Apr 13, 2010·Mutation Research·Cliona M McHaleMartyn T Smith
Jan 9, 2010·Chemico-biological Interactions·Clifford P Weisel
Feb 22, 2012·Critical Reviews in Toxicology·Christophe P StoveWilly E Lambert
Jul 25, 2014·The Science of the Total Environment·Stuart Batterman, Sergei Chernyak
Feb 21, 2019·Analytical and Bioanalytical Chemistry·Yukiko YanoStephen Rappaport
Jan 9, 2020·Current Opinion in Pediatrics·Lauren M PetrickWilliam E Funk
Oct 21, 2016·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·M Ariel Geer WallaceJoachim D Pleil
May 28, 2019·Toxics·George W Preston, David H Phillips
Jun 3, 2020·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Philip J Lupo, Logan G Spector
Nov 22, 2020·Mitochondrion·Chelsea AndersonHazel B Nichols
Jan 29, 2021·Environmental Research·Dana Boyd BarrElaine M Faustman
Mar 20, 2021·Environmental Research·Jeanette A StingoneJudit Marsillach
Feb 6, 2010·Chemical Research in Toxicology·Anoop Kumar, Gabriele Sabbioni
Jun 10, 2021·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Philip J LupoMichael E Scheurer
Sep 22, 2017·Chemical Research in Toxicology·Gabriele Sabbioni

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