Hemoglobin-induced contraction of pig pulmonary veins

The Journal of Laboratory and Clinical Medicine
S M MuldoonV W Macdonald

Abstract

The effects of hemoglobin Ao (HbAo), alpha alpha cross-linked hemoglobin (alpha alphaHb), cyanomet alpha alpha cross-linked hemoglobin (cyanomet alpha alphaHb), and human serum albumin (HSA) were compared under basal conditions and during relaxation with acetylcholine (ACh), sodium nitroprusside (SNP), and papaverine (PAP) in porcine pulmonary veins. Isometric tension changes were recorded in isolated rings (3 to 4 mm) that were suspended in Krebs solution bubbled with 95% O2/5% CO2. Increasing concentrations of HbAo and alpha alphaHb (10(-9) - 3 x 10(-6) mol/L) caused concentration-dependent increases in tension that reached a maximum of 4.20 +/- 0.3 gm and 3.78 +/- 0.6 gm, respectively. Cyanomet alpha alphaHb and HSA (10(-9) - 3 x 10(-6) mol/L) did not cause significant increases in tension. The maximum responses to HbAo and alpha alphaHb were significantly increased during relaxation with ACh and SNP but not with PAP. In contrast, SNP (10(-4) mol/L) and PAP (10(-5) mol/L), but not ACh, reversed contractions induced by HbAo and alpha alphaHb. These studies support the concept that hemoglobin-induced vascular contraction is primarily mediated by inactivation of the vasodilator nitric oxide in vitro. We suggest that this mechan...Continue Reading

Citations

Mar 3, 2004·Free Radical Biology & Medicine·Steven Deem
Jan 31, 2004·The Journal of Thoracic and Cardiovascular Surgery·D C H ChengA Vuylsteke
Jul 11, 1998·Critical Care Medicine·M P Fink
Sep 24, 2004·Transfusion·Paul W Buehler, Abdu I Alayash
Nov 10, 2007·Journal of Veterinary Pharmacology and Therapeutics·P PawsonF J Dowell
Oct 22, 2008·Transfusion·John R Hess
May 11, 1999·The Journal of Laboratory and Clinical Medicine·M A LedvinaS Muldoon
Mar 1, 2000·The Journal of Laboratory and Clinical Medicine·H W Kim, A G Greenburg
Mar 1, 1997·The Journal of Laboratory and Clinical Medicine·J L HartS M Muldoon
May 5, 1999·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·W SeftonS Muldoon
Feb 13, 2001·Anesthesia and Analgesia·M R ForrestD Royston
Sep 18, 2002·Journal of Applied Physiology·Thomas C RestaMichael P Doyle
Oct 29, 2004·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·H W KimA G Greenburg
Nov 16, 2001·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·H W KimA G Greenburg
Sep 13, 2005·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Seetharama A AcharyaPaul K Smith

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