Hemolymph amino acid analysis of individual Drosophila larvae

Analytical Chemistry
Sujeewa C PiyankarageScott A Shippy

Abstract

One of the most widely used transgenic animal models in biology is Drosophila melanogaster, the fruit fly. Chemical information from this exceedingly small organism is usually accomplished by studying populations to attain sample volumes suitable for standard analysis methods. This paper describes a direct sampling technique capable of obtaining 50-300 nL of hemolymph from individual Drosophila larvae. Hemolymph sampling performed under mineral oil and in air at 30 s intervals up to 120 s after piercing larvae revealed that the effect of evaporation on amino acid concentrations is insignificant when the sample was collected within 60 s. Qualitative and quantitative amino acid analyses of obtained hemolymph were carried out in two optimized buffer conditions by capillary electrophoresis with laser-induced fluorescence detection after derivatizing with fluorescamine. Thirteen amino acids were identified from individual hemolymph samples of both wild-type (WT) control and the genderblind (gb) mutant larvae. The levels of glutamine, glutamate, and taurine in the gb hemolymph were significantly lower at 35%, 38%, and 57% of WT levels, respectively. The developed technique that samples only the hemolymph fluid is efficient and enable...Continue Reading

References

Feb 25, 1985·Life Sciences·L MilakofskyW H Vogel
Jul 1, 1974·Neuropharmacology·A Sherman, G F Gebhart
Feb 23, 1981·Life Sciences·R P Shank, M H Aprison
Jul 13, 2002·Electrophoresis·David G McLarenDavid D Y Chen
Oct 24, 2002·Journal of Neuroscience Methods·Sumith KottegodaScott A Shippy
Jul 19, 2003·Current Opinion in Neurology·Joshua M ShulmanMel B Feany
Feb 12, 2004·Insect Biochemistry and Molecular Biology·Alan S RobinsonPeter W Atkinson
Sep 7, 2004·Electrophoresis·Kongthong Thongkhao-OnScott A Shippy
Oct 27, 2005·Molecular Interventions·Alicia M Celotto, Michael J Palladino
Jan 5, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hrvoje AugustinDavid E Featherstone

❮ Previous
Next ❯

Citations

Oct 23, 2008·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Kaiyun ChenDavid E Featherstone
May 20, 2008·Analytical Chemistry·Vratislav KostalEdgar A Arriaga
Jan 20, 2010·ACS Chemical Neuroscience·David E Featherstone
Apr 21, 2011·Disease Models & Mechanisms·Anthony EdwardsTin Tin Su
Feb 25, 2014·Journal of Insect Physiology·Heath A MacMillan, Bryon N Hughson
Nov 6, 2013·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Clara de CastroRobin L Cooper
Feb 24, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Qi ZengScott A Shippy
Jan 30, 2010·Progress in Neurobiology·Tara N Edwards, Ian A Meinertzhagen
Jul 7, 2011·Glia·David E Featherstone
Jan 18, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Qi ZengScott A Shippy
May 12, 2009·Communicative & Integrative Biology·David E FeatherstoneYael Grosjean
Dec 4, 2014·Analytica Chimica Acta·Srivani BorraScott A Shippy
Apr 27, 2012·Analytical Chemistry·Sujeewa C PiyankarageScott A Shippy
Apr 5, 2018·The Journal of Experimental Biology·Jacob B CampbellJon F Harrison
Nov 15, 2018·Journal of Fungi·Patrícia Canteri de SouzaRicardo Sergio Almeida
Dec 17, 2009·Electrophoresis·Simona ViglioPaolo Iadarola
Jan 16, 2020·Nature Reviews. Molecular Cell Biology·Grace Y Liu, David M Sabatini
Jun 1, 2015·Chemical Science·Anumita Saha-ShahLane A Baker
Aug 3, 2017·Cell Metabolism·Rachel L Wolfson, David M Sabatini
Apr 13, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Monique A MakosMichael L Heien
Mar 10, 2018·Analytical Chemistry·Marissa R CabayScott A Shippy
Feb 8, 2018·ACS Chemical Neuroscience·Mimi ShinB Jill Venton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.