Hemolysate-induced expression of intercellular adhesion molecule-1 and monocyte chemoattractant protein-1 expression in cultured brain microvascular endothelial cells via through ROS-dependent NF-kappaB pathways.

Cellular and Molecular Neurobiology
Hua LuHong-Xia Yin

Abstract

In order to determine the possible effects of hemolysate on brain microvascular endothelial cells (BMECs), we examined the effects of hemolysate on the expression of intercellular adhesion molecule-1 (ICAM-1) and monocyte chemoattractant protein-1 (MCP-1), generation of reactive oxygen species (ROS), and NF-kappaB activation in rat BMECs. Hemolysate induced the expression of ICAM-1 and MCP-1 in endothelial cells. In addition, hemolysate stimulated nuclear translocation of the p65 subunit of NF-kappaB, and NF-kappaB DNA-binding activity in BMECs. Furthermore, hemolysate increased ROS generation, and hemolysate-induced ICAM-1and MCP-1 expression and NF-kappaB activation were abrogated in the presence of the direct scavenger of ROS. Taken together, our results indicate that hemolysate can induce inflammatory responses that increase expression of ICAM-1 and MCP-1, through ROS-dependent NF-kappaB activation in BMECs.

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Citations

Nov 27, 2015·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Xiu-Li YangByung-Woo Yoon
Nov 28, 2012·European Journal of Pharmacology·Nouredine Behloul, Guanzhong Wu
Oct 22, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sharon MumbyGregory J Quinlan
Dec 5, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cuifang WangJieqing Liu

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