Hemoperfusion with polymyxin B-immobilized fibers reduced the number of CD16+ CD14+ monocytes in patients with septic shock

Journal of Endotoxin Research
Hironori TsujimotoHidetaka Mochizuki

Abstract

CD16+ CD14+ monocytes dramatically increase in number in patients with severe infection. Hemoperfusion with PMX-F (direct hemoperfusion with polymyxin B immobilized fibers) has been reported to be a safe and effective treatment for patients with septic shock, although the molecular mechanism that accounts for its effectiveness is still unclear. The purpose of this study was to quantify the number of CD16+ CD14+ monocytes in patients with an intra-abdominal infection and to evaluate the effects of PMX-F treatment on clinical parameters and leukocyte surface antigen expression in these patients. Seventeen septic patients who had an intra-abdominal infection were enrolled in this study; 7 of these patients received PMX-F treatment. Peripheral blood samples were obtained immediately after admission, and were also collected from the above 7 patients before, during, and immediately after their PMX-F treatment. The expression of CD14, CD16, and Toll-like receptor (TLR)-4 on these patients' monocytes was evaluated using flow cytometry. In addition, lipopolysaccharide (LPS)-induced production of TNF-alpha and IL-1beta by these cells was measured by ELISA. Monocytic expression of CD16 and TLR-4 was significantly greater in septic patient...Continue Reading

Citations

Apr 1, 2009·Shock·Joseph CuschieriRonald V Maier
Jun 26, 2010·BMC Anesthesiology·John W SimmonsSteven E Wolf
Apr 24, 2007·Critical Care : the Official Journal of the Critical Care Forum·Dinna N CruzClaudio Ronco
Apr 24, 2009·Journal of Clinical Immunology·Philip A EfronAlan W Hemming
Mar 27, 2007·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Kenji TsushimaKazuhiko Hora
Oct 17, 2014·Internal Medicine·Raine TataraTadashi Nagai
Oct 21, 2016·Annals of Surgery·Pablo G SanchezBartley P Griffith
Dec 4, 2008·Critical Care Medicine·Ismail Cinel, Steven M Opal
Feb 19, 2008·Shock·Hironori TsujimotoHidetaka Mochizuki

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