Abstract
Mechanisms regulating extravascular coagulation in interstitial fluids of peripheral tissues are poorly understood, since measurements of hemostatic factors in these fluids are unavailable. Because lymph from a body region reflects the composition of its interstitial fluid, we measured hemostatic factors in limb lymph of rabbits both as activity and as antigen. Mean lymph-to-plasma activity ratios were the following: fibrinogen, 0.28; prothrombin, 0.26; factor X, 0.27; factor VII, 0.17; and factors V and VIII, 0.08. All lymph fibrinogen was clottable; fibrin degradation products were absent. Lymph von Willebrand factor antigen was < 10% of plasma antigen and consisted primarily of lower molecular weight multimers. Mean lymph-to-plasma activity ratio for antithrombin was 0.38 and for tissue factor pathway inhibitor the ratio was 0.40. Low levels of antithrombin-factor Xa were measurable in lymph. The data are compatible with a basal factor VIIa-tissue factor-catalyzed extravascular activation of factor X that is prevented from progressing to generation of fibrin in limb interstitial fluid and lymph by low levels of factor VIII and factor V and by the inhibitory activity of antithrombin and tissue factor pathway inhibitor.
References
Dec 1, 1977·Scandinavian Journal of Clinical and Laboratory Investigation·W L OlszewskiH Lukasiewicz
Feb 1, 1977·Acta Physiologica Scandinavica·A EngesetL Theodorsen
Aug 15, 1992·Thrombosis Research·B J Warn-Cramer, S L Maki
Aug 15, 1990·Thrombosis Research·T A WarrS I Rapaport
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R Bach, D B Rifkin
Jul 15, 1990·Thrombosis Research·R A FleckN Varki
Mar 1, 1985·Science·H F DvorakJ McDonagh
Sep 1, 1965·The American Journal of the Medical Sciences·L J StutmanG Y Shinowara
Jan 1, 1967·The American Journal of the Medical Sciences·L ChrobákD Hnízdová
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D D WagnerV J Marder
Oct 1, 1995·American Journal of Hematology·P ChangC M Kessler
Jan 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·B J Warn-Cramer, S I Rapaport
Nov 1, 1993·The Journal of Clinical Investigation·A ZivelinS I Rapaport
Feb 1, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·S I RapaportB J Warn-Cramer
Apr 1, 1957·Acta Haematologica·A CLAUSS
Oct 1, 1960·The American Journal of Physiology·R D LANGDELLW S GIBSON
Jan 1, 1963·Scandinavian Journal of Clinical and Laboratory Investigation·R BLOMSTRANDO DAHLBACK
Apr 1, 1955·Quarterly Journal of Experimental Physiology and Cognate Medical Sciences·G C COURTICE, B MORRIS
Citations
Dec 1, 2009·Journal of Thrombosis and Haemostasis : JTH·R GopalakrishnanL V M Rao
Jul 16, 2016·Advances in Urology·Johannes-J KarschJürgen Breul
May 25, 2021·Archives of Plastic Surgery·Jeffrey C Y ChanHung-Chi Chen