Hemostatic profile and safety of pooled cryoprecipitate up to 120 hours after thawing

Transfusion
Parvez M LokhandwalaAaron A R Tobian

Abstract

AABB standards state that cryoprecipitate should be transfused within 4 to 6 hours after thawing. We evaluated coagulation factor levels and sterility of thawed pooled cryoprecipitate to assess whether shelf life can be safely extended. Donor cryoprecipitate pools (n = 20, 10 group A, 10 group O) were held at ambient temperature and sampled at 0, 4, 8, 24, 48, 72, 96, and 120 hours post-thawing for fibrinogen, Factor (F)VIII, and von Willebrand factor (vWF) levels. Samples were tested at 0 and 120 hours for sterility (BacT/Alert system). Sixty additional cryoprecipitate pools were evaluated after 72 hours. Longitudinal differences in component levels were determined by linear fixed-effects regression. Group O cryoprecipitate had significantly lower FVIII (p = 0.002) and vWF activity (p = 0.006) compared to group A at 0 hours, but were not statistically different in fibrinogen levels (p = 0.33). Fibrinogen levels were stable over 5 days: 501 ± 81 mg/unit (mean ± standard deviation) at 0 hours to 506 ± 102 mg/unit at 120 hours (p = 0.73). Similarly, there was no decline in vWF activity: 200 ± 53 IU/unit at 0 hours to 209 ± 57 IU/unit at 120 hours (p = 0.084). The FVIII activity significantly declined on average by 9.6 IU (95% con...Continue Reading

References

Apr 20, 1979·JAMA : the Journal of the American Medical Association·P M Ness, H A Perkins
Dec 4, 1980·The New England Journal of Medicine·P A JansonD Deykin
Oct 14, 2005·Critical Care : the Official Journal of the Critical Care Forum·David S Kauvar, Charles E Wade
Nov 3, 2007·Current Opinion in Critical Care·Karim BrohiRoss A Davenport
Nov 18, 2008·Statistics in Medicine·Joseph C GardinerLee Anne Roman
Feb 22, 2012·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Anna EttingerRaymond P Goodrich
Mar 29, 2014·Critical Care : the Official Journal of the Critical Care Forum·Jostein S HagemoChristine Gaarder
Mar 29, 2016·Transfusion·Laura GreenRebecca Cardigan

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Citations

May 24, 2020·American Journal of Clinical Pathology·Robert L KruseEric A Gehrie
Jun 27, 2019·Journal of Thrombosis and Haemostasis : JTH·Kie HoriokaKeiko Shimizu
Oct 22, 2020·CJEM·Barto NascimentoLuis Teodoro Da Luz
Nov 17, 2020·Military Medicine·Henry T Peng, Andrew Beckett

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