Hensin, a new collecting duct protein involved in the in vitro plasticity of intercalated cell polarity

The Journal of Clinical Investigation
Jiro TakitoQais Al-Awqati

Abstract

Two forms of intercalated cells are present in kidney collecting tubules, the alpha cell has apical endocytosis, apical H+-ATPase and basolateral band 3, while beta cells have reversed polarity of these proteins and no apical endocytosis. When a beta cell line was seeded at high density, it changed into the alpha form. We previously showed that a partially purified 230 kD extracellular matrix protein of high density cells was able to retarget band 3 from apical to basolateral domains and stimulated apical endocytosis in vitro (Van Adelsberg, J., J.C. Edwards, J. Takito, B. Kiss, and Q. Al-Awqati. 1994. Cell. 76:1053-1061). We now purify this protein, which was named hensin, to near homogeneity and find that it belongs to the macrophage scavenger receptor cysteine rich (SRCR) family. An antibody, generated against a fusion protein made from a partial cDNA recognized a 230-kD protein in rabbit kidney and in the intercalated cell line. In vitro, the hensin antibody inhibited expression of apical endocytosis. Hensin was secreted in a polarized manner and bound to the basolateral membrane and extracellular matrix. Immunohistochemistry of the kidney showed that it was expressed only in collecting tubules. Double immunofluorescence wi...Continue Reading

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Citations

Mar 12, 2009·Pflügers Archiv : European journal of physiology·Carsten A WagnerNilufar Mohebbi
Nov 14, 1997·Immunology Today·A AruffoJ Bajorath
Nov 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Xiaobo GaoQais Al-Awqati
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