Heparan sulfate 6-O-sulfotransferase 3 is involved in bone marrow mesenchymal stromal cell osteogenic differentiation‍

Biochemistry. Biokhimii︠a︡
Shancheng ZhaoJinghua Chen

Abstract

The roles of sugar chains such as heparan sulfate (HS) in stem cell self-renewal and differentiation are poorly understood. HS is a sugar chain with linear sulfated polyanionic disaccharide repeating structures that interact with many proteins, including structural proteins in the extracellular matrix and growth factors and their receptors. Thus, unraveling the role of HS in stem cell self-renewal and differentiation could provide new insights and technical routes in clinical stem cell applications. Here, we purified rat bone marrow mesenchymal stromal cells (BMMSCs) by density gradient centrifugation, analyzed mesenchymal stromal cell surface stemness marker expression by flow cytometry, and identified the sulfotransferases responsible for sulfation ester modification of HS. An osteogenic differentiation model was established by chemical induction reagents and confirmed via alkaline phosphatase (ALP) activity detection and the expression of the osteogenic differentiation markers Runx2 and Ocn. The expression profiles of HS sulfotransferases in rat BMMSCs before and after osteogenic induction were detected by RT-PCR and Western blot. Cell spheroids were formed in both control and osteogenic culture systems when BMMSCs were grow...Continue Reading

References

Apr 1, 1992·Calcified Tissue International·D D DeanB D Boyan
Sep 1, 1985·Calcified Tissue International·D D Dziewiatkowski, L L Majznerski
Dec 1, 1995·Bone·F BlanquaertJ P Caruelle
Feb 2, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D M Ornitz
Jul 6, 2000·The Orthopedic Clinics of North America·S N KhanJ M Lane
Aug 31, 2000·Developmental Biology·S Paine-SaundersS Saunders
Aug 21, 2003·Biochemical and Biophysical Research Communications·Atsushi IrieYutaka Sanai
Mar 24, 2004·Cell·Elaine FuchsGeraldine Guasch
May 12, 2005·The International Journal of Biochemistry & Cell Biology·Simon M Cool, Victor Nurcombe
Mar 4, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Rebecca A JacksonSimon M Cool
Jan 30, 2007·Biomaterials·Emma Luong-VanSimon Cool
Aug 30, 2007·Critical Reviews in Eukaryotic Gene Expression·Victor Nurcombe, Simon M Cool
Nov 6, 2007·Matrix Biology : Journal of the International Society for Matrix Biology·Benjamin MüllerChristian Götting
Aug 12, 2008·Stem Cells and Development·Christian DombrowskiSimon M Cool
Jun 23, 2010·Human Gene Therapy·Pierre Charbord
Jan 27, 2011·Biochemical Society Transactions·Raymond A A SmithCatherine L R Merry
Nov 4, 2011·Applied Microbiology and Biotechnology·Ujjwal BhaskarRobert J Linhardt
Aug 10, 2012·Nature·David Cyranoski

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