Heparan sulfate is an important mediator of Ebola virus infection in polarized epithelial cells

Virology Journal
Manasi TamhankarJean L Patterson

Abstract

Currently, no FDA-approved vaccines or treatments are available for Ebola virus disease (EVD), and therapy remains largely supportive. Ebola virus (EBOV) has broad tissue tropism and can infect a variety of cells including epithelial cells. Epithelial cells differ from most other cell types by their polarized phenotype and barrier function. In polarized cells, the apical and basolateral membrane domains are demarcated by tight junctions, and specialized sorting machinery, which results in a difference in composition between the two membrane domains. These specialized sorting functions can have important consequences for viral infections. Differential localization of a viral receptor can restrict virus entry to a particular membrane while polarized sorting can lead to a vectorial virus release. The present study investigated the impact of cell polarity on EBOV infection. Characteristics of EBOV infection in polarized cells were evaluated in the polarized Caco-2 model grown on semipermeable transwells. Transepithelial resistance (TEER), which is a function of tight junctions, was used to assess epithelial cell polarization. EBOV infection was assessed with immunofluorescence microscopy and qPCR. Statistical significance was calcu...Continue Reading

References

Jun 29, 2000·Annual Review of Biochemistry·M BernfieldM Zako
Jul 10, 2001·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·J M Anderson
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 1, 2003·Biophysical Journal·Vivian W Tang, Daniel A Goodenough
Apr 23, 2003·Virology·Helga KroschewskiChristian W Mandl
Jul 15, 2006·PLoS Pathogens·Christopher B BuckJohn T Schiller
Apr 18, 2008·Journal of Virology·Douglas E DyllaPaul B McCray
Sep 13, 2008·American Journal of Respiratory and Critical Care Medicine·Umadevi SajjanMarc B Hershenson
Jan 15, 2010·Journal of Virology·Katrin SchlieWolfgang Garten
Jun 22, 2011·Cold Spring Harbor Perspectives in Biology·Stephane SarrazinJeffrey D Esko
Apr 25, 2012·Cell Research·Xinwang CaoKai Simons
May 4, 2012·Cold Spring Harbor Perspectives in Biology·Luke Martin McCaffrey, Ian G Macara
Sep 28, 2012·Journal of Virology·S M de BoerB J Bosch
Jan 11, 2013·Journal of Virology·Beatriz SalvadorGraham Simmons
May 24, 2013·Journal of Virology·Sharof M TugizovJoel M Palefsky
Jan 7, 2014·Frontiers in Immunology·David Anak Simon Davis, Christopher R Parish
Nov 6, 2014·The New England Journal of Medicine·Elhadj Ibrahima BahRobert A Fowler
Mar 6, 2015·Journal of Virology·Aileen O'HearnLijun Rong
May 12, 2015·Annals of Global Health·Suresh Rewar, Dashrath Mirdha
May 15, 2015·Digestive Diseases and Sciences·Nisha Sharma, Mitchell S Cappell
Nov 28, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Andrew J WilsonMichael Jacobs
Jan 9, 2016·The European Respiratory Journal·Kirsty R ShortThijs Kuiken
Jul 23, 2016·The Journal of Infectious Diseases·Pauline VetterLaurent Kaiser
Sep 2, 2016·Virology Journal·Lisa HenßBarbara S Schnierle

❮ Previous
Next ❯

Citations

Aug 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Daniel P Otto, Melgardt M de Villiers
Aug 28, 2020·Open Biology·Vanessa WaznyChristine Cheung
Dec 8, 2019·Viruses·Meng HuZongqiang Cui
Aug 10, 2019·Virology Journal·Manasi Tamhankar, Jean L Patterson
Dec 29, 2020·PLoS Neglected Tropical Diseases·Hector MorenoStefan Kunz
Dec 29, 2020·Carbohydrate Polymers·Chen ShiYu Zhang
Jan 26, 2021·Journal of the Neurological Sciences·Olukemi AdekanmbiSulaiman Lakoh
Jan 27, 2021·Science Bulletin·Wei HaoZhongping Tan
Apr 15, 2021·Seminars in Thrombosis and Hemostasis·Raghuram KogantiDeepak Shukla
Aug 31, 2021·Cardiovascular Drugs and Therapy·Antonio Vitiello, Francesco Ferrara
Oct 15, 2021·Journal of the American Chemical Society·Xiangzhao AiLiangfang Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
fluorescence microscopy
glycosylation

Software Mentioned

GraphPad
GraphPad Prism
NIS Elements Imaging

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.