Heparan sulfate regulates the number and centrosome positioning of Drosophila male germline stem cells

Molecular Biology of the Cell
Daniel C LevingsHiroshi Nakato

Abstract

Stem cell division is tightly controlled via secreted signaling factors and cell adhesion molecules provided from local niche structures. Molecular mechanisms by which each niche component regulates stem cell behaviors remain to be elucidated. Here we show that heparan sulfate (HS), a class of glycosaminoglycan chains, regulates the number and asymmetric division of germline stem cells (GSCs) in the Drosophila testis. We found that GSC number is sensitive to the levels of 6-O sulfate groups on HS. Loss of 6-O sulfation also disrupted normal positioning of centrosomes, a process required for asymmetric division of GSCs. Blocking HS sulfation specifically in the niche, termed the hub, led to increased GSC numbers and mispositioning of centrosomes. The same treatment also perturbed the enrichment of Apc2, a component of the centrosome-anchoring machinery, at the hub-GSC interface. This perturbation of the centrosome-anchoring process ultimately led to an increase in the rate of spindle misorientation and symmetric GSC division. This study shows that specific HS modifications provide a novel regulatory mechanism for stem cell asymmetric division. The results also suggest that HS-mediated niche signaling acts upstream of GSC divisio...Continue Reading

References

Mar 21, 1998·Current Biology : CB·M Van DorenR Lehmann
Nov 6, 2002·Biochimica Et Biophysica Acta·Hiroshi Nakato, Koji Kimata
Mar 7, 2003·Development·Momoko FujiseHiroshi Nakato
Apr 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Toshie Kai, Allan Spradling
Sep 13, 2003·Science·Yukiko M YamashitaMargaret T Fuller
Dec 6, 2003·Science·Sean E McGuireRonald L Davis
Feb 11, 2005·Journal of Cell Science·Yukiko M YamashitaD Leanne Jones
Sep 13, 2006·The Journal of Cell Biology·Keisuke KamimuraHiroshi Nakato
Jan 27, 2007·Science·Yukiko M YamashitaMargaret T Fuller
Apr 27, 2007·Nature·Joseph R BishopJeffrey D Esko
Dec 7, 2007·Developmental Biology·Takuya AkiyamaHiroshi Nakato
Jan 23, 2008·The Journal of Cell Biology·Yukiko M Yamashita, Margaret T Fuller
Oct 17, 2008·Nature·Jun ChengYukiko M Yamashita
Jul 7, 2009·Annual Review of Cell and Developmental Biology·Shenghui HeSean J Morrison
Dec 2, 2009·The Journal of Cell Biology·Yoshiki HayashiHiroshi Nakato
Feb 26, 2010·Cold Spring Harbor Perspectives in Biology·Yukiko M YamashitaAlan J Hunt
Jul 20, 2010·Developmental Biology·Adam KleinschmitHiroshi Nakato
Sep 3, 2010·Progress in Molecular Biology and Translational Science·Hiroko Habuchi, Koji Kimata
Sep 8, 2010·Current Opinion in Cell Biology·Hebao Yuan, Yukiko M Yamashita
Apr 2, 2011·The Journal of Biological Chemistry·Katsufumi DejimaHiroshi Nakato
Jun 23, 2011·Development·Margaret de Cuevas, Erika L Matunis
Aug 3, 2011·Developmental Biology·Alexandre WojcinskiBruno Glise
Oct 9, 2012·Cell Stem Cell·Mayu Inaba, Yukiko M Yamashita
Oct 25, 2012·Development·Yoshiki HayashiDouglas A Harrison
Jan 10, 2013·The Journal of Biological Chemistry·Adam KleinschmitHiroshi Nakato
Jan 23, 2013·The Journal of Biological Chemistry·Katsufumi DejimaHiroshi Nakato
Jan 28, 2014·Cellular and Molecular Bioengineering·Viktoria SalzmannYukiko M Yamashita
Oct 26, 2014·Development·Katsufumi DejimaAndrew D Chisholm

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