Heparanase and a synthetic peptide of heparan sulfate-interacting protein recognize common sites on cell surface and extracellular matrix heparan sulfate.

The Journal of Biological Chemistry
D MarchettiD D Carson

Abstract

Heparanase is an endo-beta-D-glucuronidase that degrades the glycosaminoglycan chains of heparan sulfate (HS) proteoglycans at specific sites. Elevated levels of heparanase are associated with the metastatic potential of melanoma and other types of tumor cells. We previously reported heparanase degradation of cell surface HS subpopulations of the human adenocarcinoma cell line RL95. In the present study, heparanase activity was examined on RL95 cell surface HS subpopulations in the presence of a synthetic peptide (CRPKAKAKAKAKDQTK) of heparin/heparan sulfate-interacting protein (HIP; Liu, S., Smith, S. E., Julian, J., Rohde, L. H., Karin, N. J., and Carson, D. D. (1996) J. Biol. Chem. 271, 11817-11823). Heparanase digestion generated HS fragments from cell surface- or extracellular matrix-derived HS of approximately 25 and 9 kDa, respectively. In contrast, HS of various size classes isolated from proteoglycans secreted or released by RL95 and endothelial cells in culture were not susceptible to heparanase digestion. Incubation of heparanase-containing melanoma cellular extracts or partially purified heparanase preparations with cell surface- or ECM-derived HS and HIP peptide, but not a scrambled sequence of this peptide or othe...Continue Reading

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Citations

Oct 8, 2003·Pathology Oncology Research : POR·Dario MarchettiMadhuchhanda Roy
May 27, 2005·The Journal of Surgical Research·Naci KaraçalNecmettin Kutlu
Jun 1, 2001·Advances in Enzyme Regulation·D Marchetti, G L Nicolson
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Apr 15, 2000·Glycobiology·L A DempseyJ L Platt
May 12, 2004·Neuro-oncology·Yvonne DenkinsDario Marchetti
Nov 5, 2008·Journal of Cellular Biochemistry·Sonia D'SouzaDaniel D Carson
Dec 31, 2008·Journal of Cellular Biochemistry·Madhuchhanda Roy, Dario Marchetti
Mar 7, 2007·British Journal of Pharmacology·E A McKenzie
Aug 16, 2003·Differentiation; Research in Biological Diversity·Stephanie A MillerCatherine B Kirn-Safran
Mar 19, 2013·Matrix Biology : Journal of the International Society for Matrix Biology·Rachel GoldbergMichael Elkin
Apr 1, 2004·Birth Defects Research. Part C, Embryo Today : Reviews·Catherine B Kirn-SafranDaniel D Carson
Feb 6, 2003·International Journal of Cancer. Journal International Du Cancer·Dario MarchettiMadhuchhanda Roy
Sep 17, 1998·The Journal of Biological Chemistry·D E HokeD D Carson
Jul 8, 2018·Journal of Computer-aided Molecular Design·Hemavathy Nagarajan, Umashankar Vetrivel

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