Heparanase-enhanced shedding of syndecan-1 by myeloma cells promotes endothelial invasion and angiogenesis.

Blood
Anurag PurushothamanRalph D Sanderson

Abstract

Heparanase enhances shedding of syndecan-1 (CD138), and high levels of heparanase and shed syndecan-1 in the tumor microenvironment are associated with elevated angiogenesis and poor prognosis in myeloma and other cancers. To explore how the heparanase/syndecan-1 axis regulates angiogenesis, we used myeloma cells expressing either high or low levels of heparanase and examined their impact on endothelial cell invasion and angiogenesis. Medium conditioned by heparanase-high cells significantly stimulated endothelial invasion in vitro compared with medium from heparanase-low cells. The stimulatory activity was traced to elevated levels of vascular endothelial growth factor (VEGF) and syndecan-1 in the medium. We discovered that the heparan sulfate chains of syndecan-1 captured VEGF and also attached the syndecan-1/VEGF complex to the extracellular matrix where it then stimulated endothelial invasion. In addition to its heparan sulfate chains, the core protein of syndecan-1 was also required because endothelial invasion was blocked by addition of synstatin, a peptide mimic of the integrin activating region present on the syndecan-1 core protein. These results reveal a novel mechanistic pathway driven by heparanase expression in mye...Continue Reading

References

Apr 22, 1994·Science·P C BrooksD A Cheresh
Jun 29, 2000·Annual Review of Biochemistry·M BernfieldM Zako
Jan 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Dongfang LiuRam Sasisekharan
Jul 2, 2002·Blood·Yang YangRalph D Sanderson
Nov 25, 2003·The Journal of Biological Chemistry·Jane ReilandDario Marchetti
Dec 4, 2003·Biochemical Society Transactions·I Zachary
Oct 14, 2004·The Journal of Cell Biology·DeannaLee M BeauvaisAlan C Rapraeger
Jan 11, 2005·British Journal of Haematology·Niels Frost AndersenNiels Abildgaard
Apr 12, 2006·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Victoria DovinerYoav Sherman
May 9, 2006·Developmental Cell·Lars JakobssonLena Claesson-Welsh
Aug 12, 2006·The International Journal of Biochemistry & Cell Biology·Neta IlanIsrael Vlodavsky
Aug 29, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Constance Y Fears, Anne Woods
Feb 15, 2007·The Journal of Investigative Dermatology·Sabine A EmingJeffrey M Davidson
Feb 27, 2007·Trends in Cell Biology·Bushra Gorsi, Sally E Stringer
Mar 10, 2007·The Journal of Biological Chemistry·Yang YangRalph D Sanderson
Apr 25, 2007·Pediatric Hematology and Oncology·Rahul NaithaniSanjay Sharma
Jul 21, 2007·Circulation Research·Ganapati H MahabeleshwarTatiana V Byzova
Aug 30, 2007·Critical Reviews in Eukaryotic Gene Expression·Victor Nurcombe, Simon M Cool
Oct 24, 2007·Nature Chemical Biology·Martha L Escobar GalvisJin-Ping Li
Aug 12, 2008·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Lukas J A C HawinkelsCornelis F M Sier
Sep 25, 2008·The Journal of Biological Chemistry·Anurag PurushothamanRalph D Sanderson
Dec 31, 2008·Journal of Cellular Biochemistry·Madhuchhanda Roy, Dario Marchetti
Mar 24, 2009·PloS One·Ligong Chen, Ralph D Sanderson
Jul 15, 2009·The Journal of Biological Chemistry·Yekaterina B KhotskayaRalph D Sanderson

❮ Previous
Next ❯

Citations

Feb 11, 2011·Cancer Metastasis Reviews·Gil ArvatzIsrael Vlodavsky
Jul 8, 2011·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Nicola GiulianiSabrina Bonomini
Dec 7, 2010·The Journal of Biological Chemistry·Vishnu C RamaniRalph D Sanderson
Feb 3, 2012·The Journal of Biological Chemistry·Vishnu C RamaniRalph D Sanderson
Oct 11, 2012·The Journal of Biological Chemistry·Anurag PurushothamanRalph D Sanderson
Jan 25, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Joseph P RitchieYang Yang
Oct 3, 2013·BMC Cancer·Eloah Rabello SuarezMaria Aparecida Silva Pinhal
Aug 18, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Anna S Eriksson, Dorothe Spillmann
Mar 4, 2014·PloS One·Somali ChaterjiAaron B Baker
Jun 1, 2010·BMB Reports·Sojoong ChoiEok Soo Oh
Jan 1, 2014·Acta Pharmacologica Sinica·Yan LiuQiu-bai Li
Dec 18, 2013·Cytokine & Growth Factor Reviews·Prakash VempatiFeilim Mac Gabhann
Aug 1, 2013·Microvascular Research·Herbert H Lipowsky, Anne Lescanic
Feb 23, 2013·The Journal of Biological Chemistry·Camilla A ThompsonRalph D Sanderson
Feb 21, 2013·Expert Opinion on Therapeutic Targets·Sojoong ChoiEok-Soo Oh
Nov 13, 2015·Advanced Drug Delivery Reviews·Achilleas D TheocharisNikos K Karamanos
Feb 14, 2016·The American Journal of Pathology·Marjolein GarsenJohan van der Vlag
Sep 19, 2015·Biochimie·Sylvie Ricard-Blum, Sylvain D Vallet
May 28, 2014·Thrombosis Research·Mattias Belting
Jul 27, 2015·Cellular Signalling·Ghazal Heidari-HamedaniTünde Szatmári
Oct 16, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Maria A Gubbiotti, Renato V Iozzo
Sep 16, 2010·The FEBS Journal·Tina Manon-JensenJohn R Couchman
Feb 7, 2013·The FEBS Journal·Tina Manon-JensenJohn R Couchman
Feb 5, 2013·The FEBS Journal·Vishnu C RamaniRalph D Sanderson
Feb 13, 2013·The FEBS Journal·Amichay MeirovitzMichael Elkin
Jan 4, 2013·The FEBS Journal·Anna WadeJoanna J Phillips
Dec 16, 2010·Journal of Cellular and Molecular Medicine·Renato V Iozzo, Ralph D Sanderson
Jan 5, 2011·Biomedical Chromatography : BMC·Christina J MalavakiNikos K Karamanos

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Angiogenesis Inhibitors to Treat Cancer

Cancer treatments including angiogenesis inhibitors prevent tumor cells from receiving nutrients and oxygen. Here is the latest research on angiogenesis inhibitors for the treatment of cancer.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.