PMID: 8999930Jan 24, 1997Paper

Heparanases produce distinct populations of heparan sulfate glycosaminoglycans in Chinese hamster ovary cells

The Journal of Biological Chemistry
K J Bame, K Robson

Abstract

Once internalized, cell-associated heparan sulfate proteoglycans are degraded to short glycosaminoglycans by the action of endoglycosidases or heparanases. We have begun to address the question of how many heparanases are responsible for this process by analyzing short heparan sulfate chains produced in vivo by Chinese hamster ovary (CHO) cell heparanases. Short heparan sulfate chains were purified from CHO cells and labeled at the reducing end with [3H]NaBH4. Hydrolysis of the chains to monosaccharides and analysis of the 3H-sugar alcohols indicate that heparanase activities in CHO cells are endo-beta-glucuronidases. The modification state of the heparanase-derived glycosaminoglycans was examined by treating the [3H]heparan sulfate chains with nitrous acid or bacterial heparin lyases, which cut the chain at specific sequences, and analyzing the products by P2 gel filtration chromatography. Two populations of short chains were identified that differ in the extent of modification on the nonreducing side of the heparanase cleavage site. One class of chains is unmodified for at least 9 residues from the reducing end, while the other group has a modified domain within 3-7 residues from the heparanase cleavage site. Our results sugg...Continue Reading

References

Sep 7, 1976·Biochemistry·J E Shively, H E Conrad
Oct 1, 1979·Hoppe-Seyler's Zeitschrift für physiologische Chemie·U Klein, K von Figura
Apr 1, 1992·The International Journal of Biochemistry·J T GallagherM Lyon
Jan 1, 1991·Annual Review of Biochemistry·L Kjellén, U Lindahl
Jan 2, 1991·International Journal of Cancer. Journal International Du Cancer·R LaskovI Vlodavsky
Jun 1, 1986·The Biochemical Journal·J T GallagherW P Steward
Feb 1, 1988·Journal of Cellular Biochemistry·M NakajimaG L Nicolson
Jan 1, 1974·The Biochemical Journal·M HöökP H Iverius
Nov 1, 1993·Archives of Biochemistry and Biophysics·U R DesaiR J Linhardt
Aug 17, 1993·Biochemistry·U R DesaiR J Linhardt
Jan 1, 1995·Critical Reviews in Biochemistry and Molecular Biology·S ErnstR Sasisekharan

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Citations

Aug 30, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·L A FranssonA Oldberg
Aug 24, 1999·The Biochemical Journal·C FreemanC R Parish
Sep 12, 1997·The Journal of Biological Chemistry·X BaiJ D Esko
Aug 3, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Takaomi OhkawaNoriaki Tanaka
Jun 22, 2002·The Journal of Biological Chemistry·Katarzyna A Podyma-InoueMasaki Yanagishita
Jul 21, 1998·The Journal of Biological Chemistry·D S PikasU Lindahl

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