HepaRG Maturation in Silk Fibroin Scaffolds: Toward Developing a 3D In Vitro Liver Model.

ACS Biomaterials Science & Engineering
Alycia Abbott, Jeannine M Coburn

Abstract

In vitro liver models are necessary tools for the development of new therapeutics. HepaRG cells are a commonly used cell line to produce hepatic progenitor cells and hepatocytes. This study demonstrates for the first time the suitability of 3% silk scaffolds to support HepaRG growth and differentiation. The modulus and pore size of 3% silk scaffolds were shown to be within the desired range for liver cell growth. The optimal seeding density for HepaRG cells on silk scaffolds was determined. The growth and maturation of scaffolded HepaRG cells was evaluated for 28 days, where the first 14 days of culture were a proliferation period and the last 14 days of culture were a differentiation period using dimethyl sulfoxide (DMSO) treatment. After the first 14 days of culture, the scaffolded HepaRG cells exhibited increased metabolic activity and albumin secretion compared to monolayer cultured controls and preserved these attributes through the duration of culture. Additionally, after the first 14 days of culture, the scaffolded HepaRG cells displayed a significantly reduced expression of genes associated with hepatocyte maturation. This difference in expression was no longer apparent after 28 days of culture, suggesting that the cell...Continue Reading

References

Jun 1, 1992·The Journal of Clinical Investigation·A PietrangeloD A Shafritz
Sep 1, 1981·Experimental Cell Research·K RubinB Obrink
Mar 29, 2003·Physiological Reviews·Michael Trauner, James L Boyer
Nov 15, 2003·Biomaterials·Fergal J O'BrienLorna Gibson
Feb 27, 2004·The International Journal of Artificial Organs·B CirilloG Catapano
Aug 15, 2006·Biomaterials·Yanan DuHanry Yu
Jan 24, 2007·Chemico-biological Interactions·André GuillouzoChristiane Guguen-Guillouzo
Mar 30, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Virginie CerecAnne Corlu
Jul 27, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Puneet PuriArun J Sanyal
Aug 22, 2007·Cell Biochemistry and Biophysics·Elmus G BealeClaude Forest
Nov 21, 2007·Nature Biotechnology·Salman R Khetani, Sangeeta N Bhatia
Dec 9, 2008·Biomaterials·Brian D LawrenceDavid L Kaplan
Feb 12, 2009·Journal of Gastroenterology and Hepatology·Matteo RicchiPaola Loria
Dec 22, 2010·Journal of Cell Science·Paul A Janmey, R Tyler Miller
Jan 5, 2011·Frontiers in Bioscience (Landmark Edition)·Stevan A Gonzalez, Emmet B Keeffe
Mar 11, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sébastien AnthérieuMarie-Anne Robin
Oct 1, 2011·Nature Protocols·Danielle N RockwoodDavid L Kaplan
Jul 11, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Madhushree BhattacharyaMarjo Yliperttula
Feb 5, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Patrina GunnessFozia Noor
Feb 26, 2013·Biochimica Et Biophysica Acta·Rebecca G Wells
Dec 24, 2013·Journal of the Mechanical Behavior of Biomedical Materials·Murat GuvendirenJason A Burdick
Feb 19, 2014·Toxicology and Applied Pharmacology·Alexandra RogueAndré Guillouzo
Mar 22, 2014·Nature Reviews. Molecular Cell Biology·Keisuke Ito, Toshio Suda
Dec 17, 2014·Journal of Applied Toxicology : JAT·Zenan WangHanry Yu
Jan 27, 2015·Annual Review of Pathology·Kambez H BenamDonald E Ingber
Feb 19, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·Pamela Bachour-El AzziAndré Guillouzo
May 3, 2015·Toxicology Letters·Takafumi TomidaYoshihiro Konno
May 20, 2015·ACS Biomaterials Science & Engineering·Jelena Rnjak-KovacinaDavid L Kaplan
Jul 17, 2015·Bioscience Reports·Yu TakahashiHideo Tanaka
Jul 24, 2015·Experimental Biology and Medicine·Lawrence A VernettiD Lansing Taylor
Sep 17, 2015·Scientific Reports·Ying ChenDavid L Kaplan
Oct 11, 2015·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Christine LinSalman R Khetani
Jan 8, 2016·Toxicology and Applied Pharmacology·Anaïs MichautBernard Fromenty
Feb 27, 2016·Journal of Hepatology·Zara HannounAnne Dubart-Kupperschmitt
Jun 7, 2016·Bulletin of Experimental Biology and Medicine·A V AleksandrovaD A Sakharov
Jun 15, 2016·Lab on a Chip·Arnout SchepersSangeeta Bhatia
Jul 8, 2016·Experimental Biology and Medicine·Katrin ZeilingerFanny Knöspel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Zhen-Yu WangBo Zhai
Hepatology Research : the Official Journal of the Japan Society of Hepatology
Yichiro HiguchiHiroshi Suemizu
Drug Metabolism and Disposition : the Biological Fate of Chemicals
Kajsa P Kanebratt, Tommy B Andersson
© 2021 Meta ULC. All rights reserved