PMID: 11606255Oct 19, 2001Paper

Heparin and chondroitin sulfate inhibit adenine nucleotide hydrolysis in liver and kidney membrane enriched fractions

The International Journal of Biochemistry & Cell Biology
V L VieiraMaria Rosa Schetinger

Abstract

The inhibition of adenine nucleotide hydrolysis by heparin and chondroitin sulfate (sulfated polysaccharides) was studied in membrane preparations from liver and kidney of adult rats. Hydrolysis was measured by the activity of NTPDase and 5'-nucleotidase. The inhibition of NTPDase by heparin was observed at three different pH values (6.0, 8.0 and 10.0). In liver, the maximal inhibition observed for ATP and ADP hydrolysis was about 80% at pH 8.0 and 70% at pH 6.0 and 10.0. Similarly to the effect observed in liver, heparin caused inhibition of ATP and ADP hydrolysis that reached a maximum of 70% in kidney (pH 8.0). Na(+), K(+) and Rb(+) changed the inhibitory potency of heparin, suggesting that its effects may be related to charge interaction. In addition to heparin, chondroitin sulfate also caused a dose-dependent inhibition in liver and kidney membranes. The maximal inhibition observed for ATP and ADP hydrolysis was about 60 and 50%, respectively. In addition, the hepatic and renal activity of 5'-nucleotidase was inhibited by heparin and chondroitin sulfate, except for kidney membranes where chondroitin sulfate did not alter AMP hydrolysis. On this basis, the findings indicate that glycosaminoglycans have a potential role as i...Continue Reading

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Citations

May 28, 2004·Diabetes Research and Clinical Practice·Gilberto I L LunkesMaria R C Schetinger
May 22, 2003·Thrombosis Research·Gilberto Inácio LunkesMaria Rosa Chitolina Schetinger
Mar 5, 2003·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Daniela BalzMaria Rosa Chitolina Schetinger
Sep 5, 2002·Annual Review of Physiology·Jürgen Schnermann, David Z Levine
Aug 21, 2008·Neurochemical Research·Aracélli Gnatta DornelesVânia Lúcia Loro
Sep 4, 2010·Ecotoxicology and Environmental Safety·Peili HuangZhiwei Sun
Sep 14, 2010·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Peili HuangZhiwei Sun
Jul 27, 2010·Life Sciences·Cristina Ribas FürstenauSusana Tchernin Wofchuk
Jan 2, 2016·Pharmacological Reports : PR·Ewelina KreftMirosława Szczepańska-Konkel
Jul 12, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Chong WangChun-Yuh Charles Huang
Dec 8, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Vânia Lúcia VieiraMaria Rosa Chitolina Schetinger
Nov 24, 2011·European Journal of Pharmacology·Fernanda C VuadenCarla D Bonan
Nov 20, 2014·Cell and Tissue Research·Silvia GonzalesChun-Yuh Charles Huang

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