Abstract
Herein, the synthesis and characterization of heparin-based nanocapsules (NCs) as potential drug delivery systems is described. For the synthesis of the heparin-based NCs, the versatile method of miniemulsion polymerization at the droplets interface was achieved resulting in narrowly distributed NCs with 180 nm in diameter. Scanning and transmission electron microscopy images showed clearly NC morphology. A highly negative charge density for the heparin-based NCs was determined by measuring the electro-kinetic potential. Measuring the activated clotting time demonstrated the biological intactness of the polymeric shell. The ability of heparin-based NCs to bind to antithrombin (AT III) was investigated using isothermal titration calorimetry and dynamic light scattering experiments. The chemical stability of the NCs was studied in physiological protein-containing solutions and also in medically interesting fluids such as sodium chloride 0.9%, Ringer's solution, and phosphate buffer saline using dynamic light scattering and measuring the fluorescence intensity. The impressive uptake of NCs in different cells was confirmed by fluorescence-activated cell sorting, confocal laser scanning microscopy, and transmission electron microsco...Continue Reading
References
May 1, 1986·Thrombosis Research·G Oshima, K Nagasawa
Mar 7, 1998·Life Sciences·C PassiraniD Labarre
Aug 4, 1998·Pharmaceutical Research·C PassiraniD Labarre
Aug 1, 2000·Colloids and Surfaces. B, Biointerfaces·R GrefR H Müller
Jan 22, 2003·International Journal of Andrology·Lorella BonaccorsiElisabetta Baldi
Mar 5, 2004·Acta Anaesthesiologica Scandinavica·M I HugM Weiss
May 17, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hua-Jie WangJin-Ye Wang
May 8, 2007·Journal of Materials Science. Materials in Medicine·A K Bajpai, Smitha Bhanu
Aug 1, 1995·Molecular Medicine Today·R CarrellJ Whisstock
Aug 22, 2009·Journal of Drug Targeting·M SochaP Maincent
Jan 30, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yong-Il ChungIck Chan Kwon
Jun 22, 2010·Biopolymers·Kun-Jie WangPing Xian
Nov 23, 2010·Biomacromolecules·Ying WangKaitai Yao
Oct 7, 2011·Macromolecular Bioscience·Fen RanChangsheng Zhao
Dec 14, 2011·Chemistry : a European Journal·Christian ArgyoThomas Bein
Feb 7, 2012·The International Journal of Artificial Organs·Grit BaierKatharina Landfester
Aug 1, 2012·Biomacromolecules·Grit BaierKatharina Landfester
Mar 2, 2013·Biomacromolecules·Grit BaierKatharina Landfester
Apr 24, 2013·Colloids and Surfaces. B, Biointerfaces·Darshana JainRajiv P Gude
Citations
Dec 15, 2015·Therapeutic Delivery·Chloe MartinBridgeen Callan
Jan 26, 2016·Nanoscale·S SchöttlerV Mailänder
Apr 4, 2021·Biomolecules·Aikaterini BerdiakiDragana Nikitovic