Heparin-binding epidermal growth factor (HB-EGF) may improve embryonic development and implantation by increasing vitronectin receptor (integrin alphanubeta3) expression in peri-implantation mouse embryos.

Journal of Assisted Reproduction and Genetics
Jung Jin LimMoon Kyoo Kim

Abstract

This study investigated the effects of HB-EGF on expression of integrin alphanubeta3 and implantation of embryos. Two-cell embryos were recovered and cultured with or without 10 ng/mL HB-EGF for 96h. Expression of integrin alphanubeta3 in cultured embryos was examined by real time-RT-PCR and immunofluorescence analysis; embryos were cultured with or without HB-EGF, then transferred into the uteri of pseudo-pregnant female mice in order to analyze their implantation rate. HB-EGF improved embryonic hatching and outgrowth during extended culture, and up-regulated expression of integrin alphanubeta3 in both the preimplantation embryo and outgrowing blastocyst. Also, integrin alphanubeta3 subunits were localized at the pericellular borders and cell-cell contact areas. The number of successful implantation sites of transferred HB-EGF-treated embryos in the uterus was increased when compared to number of implantation sites with non-treated controls. HB-EGF may improve implantation by accelerating expression of integrin alphanubeta3 in peri-implantation mouse embryos.

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Citations

May 8, 2008·Current Opinion in Obstetrics & Gynecology·Kevin S Richter
Feb 2, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Leann BlombergChristoph Viebahn
Nov 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Huirong XieSudhansu K Dey
Jul 22, 2015·Reproductive Biomedicine Online·Zhengyu LiTao Yi
Jul 1, 2009·Molecular Reproduction and Development·Philip JessmonD Randall Armant
Jan 8, 2008·Seminars in Cell & Developmental Biology·Jeff ReeseB C Paria

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