PMID: 3768377Nov 7, 1986Paper

Heparin-binding fragments of fibronectin are potent inhibitors of endothelial cell growth: structure-function correlations

Biochimica Et Biophysica Acta
G A HomandbergR Eisenstein

Abstract

Heparin-binding fragments derived from the amino- and carboxyl-terminal regions of human plasma fibronectin appear to be at least relatively specific potent inhibitors of the growth of bovine aortic endothelial cells in culture by as yet unknown mechanisms. In order to understand better the sites which subserve this activity, we have compared the relative potency of other major fragments of fibronectin, most of which have dissimilar properties and do not bind heparin. We have also proteolytically digested and chemically modified the most potent of these fragments, the amino-terminal 29-kDa fragment, in order to test whether structural alterations that affect heparin-binding also affect the inhibitory property. Not all chemical modifications that abolished heparin-binding also abolished endothelial cell growth. Neither an amino-terminal 20-kDa nor a carboxyl-terminal 8-kDa subfragment of the 29-kDa fragment bound heparin; however, both were as inhibitory as native 29-kDa fragment. Reduction of the disulfides of the 20-kDa and 8-kDa fragments did not abolish inhibitory activity. We therefore conclude that the activity is not strictly conformation-dependent and that although the inhibitory activity is distributed throughout the 29...Continue Reading

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·L I GoldE Pearlstein
Jul 15, 1977·International Journal of Cancer. Journal International Du Cancer·E Engvall, E Ruoslahti
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A J MillisM J Brennan
May 1, 1985·Archives of Biochemistry and Biophysics·G A HomandbergM W Mosesson
May 27, 1982·Nature·S Taylor, J Folkman
Mar 1, 1984·American Journal of Ophthalmology·G A WilliamsD Grant
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T E PetersenS Magnusson
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M D Pierschbacher, E Ruoslahti
Nov 14, 1984·Biochemical and Biophysical Research Communications·R M Robinson, J Hermans
Jan 1, 1983·Annual Review of Biochemistry·K M Yamada
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M PierschbacherE Ruoslahti
Apr 16, 1984·European Journal of Biochemistry·K SkorstengaardT E Petersen
Mar 1, 1983·Experimental Eye Research·B JacobsonS M Hasany
Jan 1, 1983·Methods in Enzymology·A FontanaM Zambonin
Apr 23, 1999·The Journal of Biological Chemistry·D A MasonS B Liggett
Oct 23, 2009·Brain : a Journal of Neurology·Lindsay E RhodesKenneth H Fischbeck
Jan 1, 1972·Methods in Enzymology·J F Riordan, B L Vallee
Jan 1, 1972·Methods in Enzymology·M J Hunter, M L Ludwig
Jan 1, 1972·Methods in Enzymology·K L Carraway, D E Koshland

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Citations

Jan 1, 1991·Pharmacology & Therapeutics·R Eisenstein
Sep 1, 1994·Pharmacology & Therapeutics·W Auerbach, R Auerbach
Mar 1, 1997·Matrix Biology : Journal of the International Society for Matrix Biology·N Burton-WursterJ N Macleod
Sep 1, 1987·The Journal of Cell Biology·A Orlidge, P A D'Amore
Jan 1, 1992·The Journal of Cell Biology·D Muir, M Manthorpe
Feb 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·M Yi, E Ruoslahti
Mar 1, 1994·Journal of Cellular Biochemistry·T VogelD D Roberts
Jul 24, 2010·Journal of Tissue Engineering and Regenerative Medicine·O A BranfordV Mudera
Mar 9, 2000·Seminars in Arthritis and Rheumatism·M L Barilla, S E Carsons
May 30, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ujjwala M WarawdekarNarendra G Mehta
Feb 1, 1990·Biological Chemistry Hoppe-Seyler·T PlanchenaultV Keil-Dlouha
Feb 1, 1990·Biological Chemistry Hoppe-Seyler·J M ImhoffV Keil-Dlouha
Nov 1, 1987·Thrombosis Research·G A Homandberg, J Kramer-Bjerke
Jul 24, 2014·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Amandine JullienneJérôme Badaut
Feb 24, 2016·Cellular and Molecular Bioengineering·Karin WangDelphine Gourdon
Nov 10, 1995·The Journal of Biological Chemistry·R M StrieterD Marriott
Jan 23, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ruben DaumKatja Schenke-Layland
Apr 24, 1999·Journal of the American Society of Nephrology : JASN·S WagaM Yokoyama

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